Weighted gene co-expression network analysis of hub genes in lung adenocarcinoma

Lung adenocarcinoma (LUAD) is a tumor with high incidence. This study aimed to identify the central genes of LUAD. LUAD were analyzed by weighted gene co-expression network (WGCNA), and differentially expressed genes (DEGs) were identified. Samples were obtained from The Cancer Genome Atlas (TCGA) a...

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Bibliographic Details
Published in:Evolutionary bioinformatics online 2021, Vol.17, p.11769343211009898-11769343211009898
Main Authors: Luo, Xuan, Feng, Lei, Xu, WenBo, Bai, XueJing, Wu, MengNa
Format: Article
Language:English
Subjects:
Adenocarcinoma
Algorithms
Calcium channels (voltage-gated)
Cancer
E-cadherin
Gene expression
Genes
Genomes
Genotypes
Interleukin 6
Lungs
miRNA
Modules
Network analysis
Original Research
Tumors
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Summary:Lung adenocarcinoma (LUAD) is a tumor with high incidence. This study aimed to identify the central genes of LUAD. LUAD were analyzed by weighted gene co-expression network (WGCNA), and differentially expressed genes (DEGs) were identified. Samples were obtained from The Cancer Genome Atlas (TCGA) and Genotype Tissue Expression (GTEx) databases and included 515 LUAD samples and 347 normal samples. The WGCNA algorithm generated a total of 10 modules. The top 2 modules (MEturquoise and MEblue) with the highest correlation to LUAD were selected. Ten Hub genes (IL6, CDH1, PECAM1, SPP1, THBS1, HGF, SNCA, CDH5, CAV1, and DLC1) were screened in the intersecting genes of DEGs and WGCNA (MEturquoise and MEblue). Only SPP1 was correlated with LUAD poor survival, indicating that SPP1 may be a key Hub gene for LUAD. The competing endogenous RNA (ceRNA) network was constructed to analyze the regulatory relationship of Hub genes, and SPP1 may be directly regulated by 4 microRNAs (miRNAs) and indirectly regulated by 49 long noncoding RNAs (lncRNAs).
ISSN:1176-9343
1176-9343
DOI:10.1177/11769343211009898