Immobilization of Isocitrate Dehydrogenase on Mesoporous Silica Foam for Carbon Dioxide Capture

Carbon capture can be realized effectively through isocitrate dehydrogenase reaction and the reaction rate was strongly affected by the environmental parameters such as pH and temperature. Enzyme immobilization was conducted to improve the enzyme stability during the capture process. By simply adsor...

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Bibliographic Details
Main Authors: Xia, Shunxiang, Zhang, Laibao, Veony, Enjelia
Format: Conference Proceeding
Language:English
Subjects:
Carbon dioxide
Carbon sequestration
Dehydrogenase
Dehydrogenases
Enzymatic activity
Enzyme activity
Enzymes
Immobilization
Isocitrate dehydrogenase
pH effects
Shear stress
Silica
Silicon dioxide
Surface stability
Temperature
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Summary:Carbon capture can be realized effectively through isocitrate dehydrogenase reaction and the reaction rate was strongly affected by the environmental parameters such as pH and temperature. Enzyme immobilization was conducted to improve the enzyme stability during the capture process. By simply adsorbing enzyme on the surface of mesoporous silica foam, enzyme stability against temperature, pH and shear stress was improved. The immobilization process can be completed in 5 mins, and 0.87 U enzyme activity was kept for each gram of immobilization material. After 10 cycles, more than 50 percent of enzyme activity remained. The reusability and improved stability made immobilized ICDH a better candidate for large-scale application of carbon capture.
ISSN:2267-1242
2555-0403
2267-1242
DOI:10.1051/e3sconf/20199304001