Korean Traditional Medicine (Jakyakgamcho-tang) Ameliorates Colitis by Regulating Gut Microbiota

The objective of this study was to examine the anti-colitis activity of Jakyakgamcho-tang (JGT) in dextran sulfate sodium (DSS)-induced colitis and explore changes of the gut microbial community using 16S rRNA amplicon sequencing and metabolomics approaches. It was found that treatment with JGT or 5...

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Bibliographic Details
Published in:Metabolites 2019-10, Vol.9 (10), p.226
Main Authors: Seo, Seung-Ho, Unno, Tatsuya, Park, Seong-Eun, Kim, Eun-Ju, Lee, Yu-Mi, Son, Hong-Seok
Format: Article
Language:English
Subjects:
Acids
Colitis
Correlation analysis
Cytokines
Dextran
Dextran sulfate
Discriminant analysis
Drug dosages
Dysbacteriosis
Experiments
Genera
Herbal medicine
Inflammation
Inflammatory bowel disease
Interleukin 12
Interleukin 6
Intestinal microflora
jakyakgamcho-tang
Lactic acid
Metabolites
Metabolomics
Microbiomes
Microbiota
Multidimensional scaling
Pharmaceutical industry
rRNA 16S
Software
Taxonomy
Traditional medicine
Tumor necrosis factor-TNF
γ-Interferon
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Summary:The objective of this study was to examine the anti-colitis activity of Jakyakgamcho-tang (JGT) in dextran sulfate sodium (DSS)-induced colitis and explore changes of the gut microbial community using 16S rRNA amplicon sequencing and metabolomics approaches. It was found that treatment with JGT or 5-aminosalicylic acid (5-ASA) alleviated the severity of colitis symptoms by suppressing inflammatory cytokine levels of IL-6, IL-12, and IFN-γ. The non-metric multidimensional scaling analysis of gut microbiome revealed that JGT groups were clearly separated from the DSS group, suggesting that JGT administration altered gut microbiota. The operational taxonomic units (OTUs) that were decreased by DSS but increased by JGT include Akkermansia and Allobaculum. On the other hand, OTUs that were increased by DSS but decreased by 5-ASA or JGT treatments include Bacteroidales S24-7, Ruminococcaceae, and Rikenellaceae, and the genera Bacteroides, Parabacteroides, Oscillospira, and Coprobacillus. After JGT administration, the metabolites, including most amino acids and lactic acid that were altered by colitis induction, became similar to those of the control group. This study demonstrates that JGT might have potential to effectively treat colitis by restoring dysbiosis of gut microbiota and host metabolites.
ISSN:2218-1989
2218-1989
DOI:10.3390/metabo9100226