Improved fermentation efficiency of S. cerevisiae by changing glycolytic metabolic pathways with plasma agitation

Production of ethanol by the yeast Saccharomyces cerevisiae is a process of global importance. In these processes, productivities and yields are pushed to their maximum possible values leading to cellular stress. Transient and lasting enhancements in tolerance and performance have been obtained by g...

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Bibliographic Details
Published in:Scientific reports 2018-05, Vol.8 (1), p.8252-13, Article 8252
Main Authors: Recek, Nina, Zhou, Renwu, Zhou, Rusen, Te’o, Valentino Setoa Junior, Speight, Robert E., Mozetič, Miran, Vesel, Alenka, Cvelbar, Uros, Bazaka, Kateryna, Ostrikov, Kostya (Ken)
Format: Article
Language:English
Subjects:
14/28
14/63
631/326
639/766/1960
Agitation
Cellular stress response
Chemical speciation
Environmental stress
Ethanol
Fermentation
Genetic engineering
Glycolysis
Hexokinase
Humanities and Social Sciences
Membrane structure
Metabolic pathways
Metabolism
Metabolites
multidisciplinary
Phenotypes
Photons
Plasma
Saccharomyces cerevisiae
Science
Science (multidisciplinary)
Yeast
Yeasts
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Summary:Production of ethanol by the yeast Saccharomyces cerevisiae is a process of global importance. In these processes, productivities and yields are pushed to their maximum possible values leading to cellular stress. Transient and lasting enhancements in tolerance and performance have been obtained by genetic engineering, forced evolution, and exposure to moderate levels of chemical and/or physical stimuli, yet the drawbacks of these methods include cost, and multi-step, complex and lengthy treatment protocols. Here, plasma agitation is shown to rapidly induce desirable phenotypic changes in S . cerevisiae after a single treatment, resulting in improved conversion of glucose to ethanol. With a complex environment rich in energetic electrons, highly-reactive chemical species, photons, and gas flow effects, plasma treatment simultaneously mimics exposure to multiple environmental stressors. A single treatment of up to 10 minutes performed using an atmospheric pressure plasma jet was sufficient to induce changes in cell membrane structure, and increased hexokinase 2 activity and secondary metabolite production. These results suggest that plasma treatment is a promising strategy that can contribute to improving metabolic activity in industrial microbial strains, and thus the practicality and economics of industrial fermentations.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-26227-5