Influence of the nano-micro structure of the surface on bacterial adhesion

Biomaterials failures are frequently associated to the formation of bacterial biofilms on the surface. The aim of this work is to study the adhesion of non motile bacteria streptococci consortium and motile Pseudomonas fluorescens. Substrates with micro and nanopatterned topography were used. The in...

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Bibliographic Details
Published in:Materials research (São Carlos, São Paulo, Brazil) São Paulo, Brazil), 2007-03, Vol.10 (1), p.11-14
Main Authors: Díaz, Carolina, Cortizo, María Cecilia, Schilardi, Patricia Laura, Saravia, Sandra Gabriela Gómez de, Mele, Mónica Alicia Fernández Lorenzo de
Format: Article
Language:English
Subjects:
bacterial adhesion
biofouling
ENGINEERING, CHEMICAL
extracellular polymeric material
MATERIALS SCIENCE, MULTIDISCIPLINARY
METALLURGY & METALLURGICAL ENGINEERING
micro
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Summary:Biomaterials failures are frequently associated to the formation of bacterial biofilms on the surface. The aim of this work is to study the adhesion of non motile bacteria streptococci consortium and motile Pseudomonas fluorescens. Substrates with micro and nanopatterned topography were used. The influence of surface characteristics on bacterial adhesion was investigated using optical and epifluorescence microscopy, scanning electron microscopy (SEM) and atomic force microscopy (AFM). Results showed an important influence of the substratum nature. On microrough surfaces, initial bacterial adhesion was less significant than on smooth surfaces. In contrast, nanopatterned samples showed more bacterial attachment than the smooth control. It was also noted a remarkable difference in morphology, orientation and distribution of bacteria between the smooth and the nanostructured substrate. The results show the important effect of substratum nature and topography on bacterial adhesion which depended on the relation between roughness characteristics dimensions and bacterial size.
ISSN:1516-1439
1980-5373
1516-1439
DOI:10.1590/S1516-14392007000100004