Establishing the relationship between subjective perception and neural responses: Insights from correlation analysis and representational similarity analysis

• Published in: NeuroImage (Orlando, Fla.) Fla.), 2024-07, Vol.295, p.120650, Article 120650
• Main Authors: Liu, Xu, Wei, Shiyu, Zhao, Xiangyue, Bi, Yanzhi, Hu, Li
• Format: Article
• Language: English
• Subjects: Adult; Auditory Perception - physiology; Behavior; Brain - physiology; Clinical trials; Correlation analysis; EEG; Electrodes; Electroencephalography - methods; Experiments; Female; Humans; Lasers; Male; Pain perception; Reliability; Representational similarity analysis; Sensory integration; Sensory perception; Sensory stimuli; Statistical analysis; Visual Perception - physiology; Young Adult
• ISSN: 1053-8119; 1095-9572; 1095-9572
• DOI: 10.1016/j.neuroimage.2024.120650

•Within-subject correlation revealed strong and reliable correlations.•Between-subject correlation showed weak correlations.•Between-subject correlation was vulnerable to the change of subject number.•RSA revealed novel findings that couldn't be detected through correlation analyses.•RSA results were sensitive to the trial number, but not to the subject number. Exploring the relationship between sensory perception and brain responses holds important theoretical and clinical implications. However, commonly used methodologies like correlation analysis performed either intra- or inter- individually often yield inconsistent results across studies, limiting their generalizability. Representational similarity analysis (RSA), a method that assesses the perception-response relationship by calculating the correlation between behavioral and neural patterns, may offer a fresh perspective to reveal novel findings. Here, we delivered a series of graded sensory stimuli of four modalities (i.e., nociceptive somatosensory, non-nociceptive somatosensory, visual, and auditory) to/near the left or right hand of 107 healthy subjects and collected their single-trial perceptual ratings and electroencephalographic (EEG) responses. We examined the relationship between sensory perception and brain responses using within- and between-subject correlation analysis and RSA, and assessed their stability across different numbers of subjects and trials. We found that within-subject and between-subject correlations yielded distinct results: within-subject correlation revealed strong and reliable correlations between perceptual ratings and most brain responses, while between-subject correlation showed weak correlations that were vulnerable to the change of subject number. In addition to verifying the correlation results, RSA revealed some novel findings, i.e., correlations between behavioral and neural patterns were observed in some additional neural responses, such as “γ-ERS” in the visual modality. RSA results were sensitive to the trial number, but not to the subject number, suggesting that consistent results could be obtained for studies with relatively small sample sizes. In conclusion, our study provides a novel perspective on establishing the relationship between behavior and brain activity, emphasizing that RSA holds promise as a method for exploring this pattern relationship in future research.