Effect of dexmedetomidine on acute lung injury in experimental ischemia–reperfusion model

• Published in: Brazilian journal of anesthesiology (Elsevier) 2017-03, Vol.67 (2), p.139-146
• Main Authors: Küçükebe, Ömer Burak, Özzeybek, Deniz, Abdullayev, Ruslan, Ustaoğlu, Adil, Tekmen, Işıl, Küme, Tuncay
• Format: Article
• Language: English
• Subjects: Acute lung injury; Acute Lung Injury - prevention & control; Adrenergic alpha-2 Receptor Agonists - administration & dosage; Adrenergic alpha-2 Receptor Agonists - pharmacology; ANESTHESIOLOGY; Animals; Dexmedetomidina; Dexmedetomidine; Dexmedetomidine - administration & dosage; Dexmedetomidine - pharmacology; Disease Models, Animal; Dose-Response Relationship, Drug; Experimental; Female; Interleukin-6 - blood; Lesão de reperfusão; Lesão pulmonar aguda; Lower Extremity - blood supply; Rats; Rats, Wistar; Reperfusion injury; Reperfusion Injury - complications; Reperfusion Injury - drug therapy; Tourniquets - adverse effects; Tumor Necrosis Factor-alpha - blood
• ISSN: 0104-0014; 1806-907X; 0104-0014
• DOI: 10.1016/j.bjane.2015.08.002

Ischemia–reperfusion injury is one of the consequences of tourniquet application for extremity surgery. The aim of the study was to establish the effect of dexmedetomidine on the acute lung injury following lower extremity experimental ischemia–reperfusion model in rats. Twenty-eight Wistar-Albino breed Rats were recruited after Ethics Committee approval and allocated into 4 groups, each with 7 subjects. Group 1 (SHAM) received only anesthesia. Group 2 (IR) had experienced 3h of ischemia and 3h of reperfusion using left lower extremity tourniquet after anesthesia application. Groups 3 (D-50) and 4 (D-100) had undergone the same procedures as in the Group 2, except for receiving 50 and 100mg·kg−1, respectively, dexmedetomidine intraperitoneally 1h before the tourniquet release. Blood samples were obtained for the analysis of tumor necrosing factor-α and interleukin-6. Pulmonary tissue samples were obtained for histological analysis. No significant difference regarding blood tumor necrosing factor-α and interleukin-6 values was found among the groups, whereas pulmonary tissue injury scores revealed significant difference. Histological scores obtained from the Group 2 were significantly higher from those in the Groups 1, 3 and 4 with p-values 0.001 for each comparison. Moreover, Group 1 scores were found to be significantly lower than those in the Groups 3 and 4 with p-values 0.001 and 0.011, respectively. No significant difference was observed between the Groups 3 and 4. Dexmedetomidine is effective in reduction of the experimental ischemia–reperfusion induced pulmonary tissue injury in rats, formed by extremity tourniquet application. A lesão de isquemia-reperfusão é uma das consequências da aplicação do torniquete em cirurgias de extremidades. O objetivo do estudo foi determinar o efeito de dexmedetomidina em lesão pulmonar aguda após modelo experimental de isquemia-reperfusão em extremidade inferior de ratos. Vinte e oito ratos albinos Wistar foram recrutados após aprovação do Comitê de Ética e alocados em quatro grupos, cada um com sete indivíduos. O Grupo 1 (Sham) recebeu apenas anestesia. O Grupo 2 (IR) foi submetido a 3 horas de isquemia e 3 horas de reperfusão com o uso de torniquete em extremidade inferior após a aplicação da anestesia. Os grupos 3 (D-50) e 4 (D-100) foram submetidos aos mesmos procedimentos do Grupo 2, exceto por receberem 50 e 100mg·kg−1 de dexmedetomidina, respectivamente, por via intraperitoneal 1 hora antes da liberação do torn