Cough associated with the detection of Mycoplasma hyopneumoniae DNA in clinical and environmental specimens under controlled conditions

The association of cough with Mycoplasma hyopneumoniae (MHP) DNA detection in specimens was evaluated under conditions in which the MHP status of inoculated and contact-infected pen mates was closely monitored for 59 days post-inoculation (DPI). Seven-week-old pigs (n = 39) were allocated to five ro...

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Published in:Porcine Health Management. 2022-01, Vol.8 (1), p.6-6, Article 6
Main Authors: Silva, Ana Paula S Poeta, Storino, Gabriel Y, Ferreyra, Franco S Matias, Zhang, Min, Fano, Eduardo, Polson, Dale, Wang, Chong, Derscheid, Rachel J, Zimmerman, Jeffrey J, Clavijo, Maria J, Arruda, Bailey L
Format: Article
Language:English
Subjects:
Air samples
Antibodies
Catheters
Deoxyribonucleic acid
DNA
Enzootic pneumonia
Fluids
Hogs
Infections
Laboratory animals
Larynx
Mycoplasma hyopneumoniae
Oral fluids
Pathogens
Surveillance
Tracheal swabs
Water samples
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Summary:The association of cough with Mycoplasma hyopneumoniae (MHP) DNA detection in specimens was evaluated under conditions in which the MHP status of inoculated and contact-infected pen mates was closely monitored for 59 days post-inoculation (DPI). Seven-week-old pigs (n = 39) were allocated to five rooms (with one pen). Rooms contained 9 pigs each, with 1, 3, 6, or 9 MHP-inoculated pigs, respectively, except Room 5 (three sham-inoculated pigs). Cough data (2 × week) and specimens, tracheal swabs (2 × week), oral fluids (daily), drinker wipes (~ 1 × week), and air samples (3 × week) were collected. At 59 DPI, pigs were euthanized, and lung and trachea were evaluated for gross and microscopic lesions. Predictive cough value to MHP DNA detection in drinker and oral fluid samples were estimated using mixed logistic regression. Following inoculation, MHP DNA was first detected in tracheal swabs from inoculated pigs (DPI 3), then oral fluids (DPI 8), air samples (DPI 10), and drinker wipes (21 DPI). MHP DNA was detected in oral fluids in 17 of 59 (Room 1) to 43 of 59 (Room 3) samples, drinker wipes in 4 of 8 (Rooms 2 and 3) to 5 of 8 (Rooms 1 and 4) samples, and air samples in 5 of 26 (Room 2) or 3 of 26 (Room 4) samples. Logistic regression showed that the frequency of coughing pigs in a pen was associated with the probability of MHP DNA detection in oral fluids (P 
ISSN:2055-5660
2055-5660
DOI:10.1186/s40813-022-00249-y