Utilisation of in vitro techniques in rescue of gene resources of meadow vetchling (Lathyrus pratensis)

In the case of poor germination of seed samples and minimal number of seedlings obtained, in vitro methods can be used to revitalise and recover the gene resource. The highest germination of meadow vetchling (Lathyrus pratensis) seeds was achieved after scarification with sulphuric acid and cultivat...

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Bibliographic Details
Published in:Czech Journal of Genetics and Plant Breeding 2003-09, Vol.39 (3), p.84-88
Main Authors: Klcova, L, Gubisova, M. (Vyskumny Ustav Rastlinnej Vyroby, Piestany (Slovak Republic))
Format: Article
Language:English
Subjects:
BIODIVERSIDAD
BIODIVERSITY
BIODIVERSITÉ
CULTIVO IN VITRO
CULTURE IN VITRO
Culture media
Cytokinins
gene resources
GENETIC RESOURCES
Germination
in vitro cultivation
IN VITRO CULTURE
In vitro methods and tests
LATHYRUS
Lathyrus pratensis
lathyrus pratensis l
meadow vetchling
MEADOWS
MICROPROPAGACIÓN
MICROPROPAGATION
Multiplication
Plant maintenance
PRADOS DE SIEGA
PRAIRIE DE FAUCHE
RECURSOS GENÉTICOS
Regeneration
REPÚBLICA ESLOVACA
RESSOURCE GÉNÉTIQUE
revitalisation
Rooting
RÉPUBLIQUE SLOVAQUE
seed germination
Seedlings
Seeds
Shoots
SLOVAK REPUBLIC
Sulfuric acid
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Summary:In the case of poor germination of seed samples and minimal number of seedlings obtained, in vitro methods can be used to revitalise and recover the gene resource. The highest germination of meadow vetchling (Lathyrus pratensis) seeds was achieved after scarification with sulphuric acid and cultivation in MS medium. The seedlings were used as a material for micropropagation. Regeneration passed through nodal segments cultivated on basal MS medium solidified with a combination of agar and phytagel. This culture medium was also suitable for the plant maintenance. An addition of cytokinin to the induction medium did not support multiplication and growth. In the basal MS medium rooted 72.5% (gene resource 62) or 42.5% (gene resource 28) of shoots. The rooting of gene resource 28 was increased to 63% by the addition of indolylbutyric acid to the culture medium. The regenerated plants were successfully transferred to the soil. This protocol can be used to rescue gene resources of this species.
ISSN:1212-1975
1212-1975
1805-9325
DOI:10.17221/3724-CJGPB