Effects of β-glucosidase and α-rhamnosidase on the Contents of Flavonoids, Ginkgolides, and Aroma Components in Ginkgo Tea Drink

• Published in: Molecules (Basel, Switzerland) Switzerland), 2019-05, Vol.24 (10), p.2009
• Main Authors: Fang, Xianying, Dong, Yurong, Xie, Yingying, Wang, Lei, Wang, Jingqiu, Liu, Yuechen, Zhao, Linguo, Cao, Fuliang
• Format: Article
• Language: English
• Subjects: anti-inflammation; anti-oxidation; anti-tumor; Aroma; aroma component; beta-Glucosidase - metabolism; Beverages - analysis; Biocompatibility; Biological activity; Cell activation; Cellobiase; Cytotoxicity; Enzymes; Flavone glycosides; flavonoid; Flavonoids; Flavonoids - metabolism; Flavor; Free radicals; Ginkgo; Ginkgo biloba - chemistry; ginkgo tea; ginkgolide; Ginkgolides; Ginkgolides - metabolism; Glucosidase; glycosidase; Glycosidases; Glycoside Hydrolases - metabolism; Glycosides; Headspace; High temperature; High-performance liquid chromatography; Inflammation; Liquid chromatography; Natural & organic foods; Odorants - analysis; Palatability; Scavenging; Tea; β-Glucosidase
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24102009

Ginkgo tea is a kind of health food produced from leaves. The market of Ginkgo tea encountered many difficulties because of its bad palatability and vague function statement. In this study, two kinds of glycosidase were used to improve the flavor of Ginkgo tea, and three kinds of bioactivities were selected to investigate the health care function of the tea infusion. The aroma components extracted by headspace absorb (HSA) method during the making of Ginkgo tea were analyzed by GC-MS. The flavonoids and ginkgolides released into the tea infusion were studied by HPLC. A combination of β-glucosidase (β-G) and α-rhamnosidase (α-R) was applied during the making of the tea. The contents of characteristic aroma components and the release of total flavonoids and ginkgolides were increased significantly by adding β-G and α-R. The composition of flavone glycosides was changed greatly. The free radical scavenging, inhibition of inflammatory cell activation, and tumor cytotoxicity activities of the tea were demonstrably improved. According to the release of active components, Ginkgo tea can be brewed repeatedly for at least three times. The enzymes used here show potential application prospects in the making of Ginkgo tea or tea drink to get higher contents of flavonoids, ginkgolides, and aroma components.