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Expression of Long Non-Coding RNA H19 in Acute Lymphoblastic Leukemia
OObjective: Long non-coding RNA (lncRNA) H19 has essential roles in growth, migration, invasion, and metastasis of most cancers. H19 dysregulation is present in a large number of solid tumors and leukemia. However, the expression level of H19 in acute lymphoblastic leukemia (ALL) has not been elucid...
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| Published in: | Cell journal (Yakhteh) 2023-01, Vol.25 (1), p.1-10 |
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| creator | Asadi, Marjan Gholampour, Mohammad Ali Kompani, Farzad Alizadeh, Shaban |
| description | OObjective: Long non-coding RNA (lncRNA) H19 has essential roles in growth, migration, invasion, and metastasis of most cancers. H19 dysregulation is present in a large number of solid tumors and leukemia. However, the expression level of H19 in acute lymphoblastic leukemia (ALL) has not been elucidated yet. The current study aimed to explore
expression in ALL patients and cell lines.
This experimental study was conducted in bone marrow (BM) samples collected from 25 patients with newly diagnosed ALL. In addition, we cultured the RPMI-8402, Jurkat, Ramos, and Daudi cell lines and assessed the effects of internal (hypoxia) and external (chemotherapy medications L-asparaginase [ASP] and vincristine [VCR]) factors on h19 expression. The expressions of
and
were performed using quantitative real-time polymerase chain reaction (qRT-PCR) method.
There was significantly increased
expression in the B-cell ALL (B-ALL, P |
| doi_str_mv | 10.22074/cellj.2022.8315 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_7e47be2e67584bac834088be470978a3</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_7e47be2e67584bac834088be470978a3</doaj_id><sourcerecordid>2768233065</sourcerecordid><originalsourceid>FETCH-LOGICAL-c370t-582e5b629a8357144a55f974e47fd23b53efa6391bcc549dd67ccfa732a2c64c3</originalsourceid><addsrcrecordid>eNpdkc1Lw0AQxRdRrKh3TxLw4qV1M_uZi1BKtYWgIHpeNptNuzXJ1mwi-t-7fqLOZYY3jx-PGYROUjwBwIJeGFvXmwlggIkkKdtBBwAgx0ymdPdnxnyEjkPY4FgcQ5rBPhoRziWmQh6g-fxl29kQnG8TXyW5b1fJjW_HM1-6ON7dTJNFmiWuTaZm6G2SvzbbtS9qHXpnktwOj7Zx-gjtVboO9virH6KHq_n9bDHOb6-Xs2k-NkTgPqYBywoOmZaEiZRSzViVCWqpqEogBSO20pxkaWEMo1lZcmFMpQUBDYZTQw7R8pNber1R2841untVXjv1IfhupXQXg9VWiQgtLFgumKSFNpJQLGURVZwJqUlkXX6ytkPR2NLYtu90_Qf6d9O6tVr5Z5VJLikREXD-Bej802BDrxoX3n-iW-uHoEBwCYRgzqL17J9144eujadSIAmWDLOURtfp70Q_Ub6_Rd4AQmKV_Q</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2830850514</pqid></control><display><type>article</type><title>Expression of Long Non-Coding RNA H19 in Acute Lymphoblastic Leukemia</title><source>Open Access: PubMed Central</source><source>Publicly Available Content Database</source><creator>Asadi, Marjan ; Gholampour, Mohammad Ali ; Kompani, Farzad ; Alizadeh, Shaban</creator><creatorcontrib>Asadi, Marjan ; Gholampour, Mohammad Ali ; Kompani, Farzad ; Alizadeh, Shaban</creatorcontrib><description>OObjective: Long non-coding RNA (lncRNA) H19 has essential roles in growth, migration, invasion, and metastasis of most cancers. H19 dysregulation is present in a large number of solid tumors and leukemia. However, the expression level of H19 in acute lymphoblastic leukemia (ALL) has not been elucidated yet. The current study aimed to explore
expression in ALL patients and cell lines.
This experimental study was conducted in bone marrow (BM) samples collected from 25 patients with newly diagnosed ALL. In addition, we cultured the RPMI-8402, Jurkat, Ramos, and Daudi cell lines and assessed the effects of internal (hypoxia) and external (chemotherapy medications L-asparaginase [ASP] and vincristine [VCR]) factors on h19 expression. The expressions of
and
were performed using quantitative real-time polymerase chain reaction (qRT-PCR) method.
There was significantly increased
expression in the B-cell ALL (B-ALL, P<0.05), T-cell ALL (T-ALL, P<0.01) patients and the cell lines. This upregulation was governed by the
and
transcription factors. We observed that increased
expression induced
expression; however,
adversely affected
expression. In addition, the results indicated that chemotherapy changed the gene expression pattern. There was a considerable decrease in
expression after exposure to chemotherapy medications; nonetheless, hypoxia induced
expression through
downregulation.
Our findings suggest that
may have an important role in pathogenesis in ALL and may act as a promising and potential therapeutic target.</description><identifier>ISSN: 2228-5806</identifier><identifier>EISSN: 2228-5814</identifier><identifier>DOI: 10.22074/cellj.2022.8315</identifier><identifier>PMID: 36680478</identifier><language>eng</language><publisher>Iran: Royan Institute of Iran</publisher><subject>Actin ; Acute lymphoblastic leukemia ; Asparaginase ; Bone marrow ; c-Myc protein ; Chemotherapy ; Gene expression ; h19 ; Hypoxia ; Hypoxia-inducible factor 1a ; L-asparaginase ; Leukemia ; lncrna ; Lymphocytes B ; Lymphocytes T ; Metastases ; Myc protein ; Non-coding RNA ; Original ; p53 Protein ; Pathogenesis ; Polymerase chain reaction ; Ribonucleic acid ; RNA ; Solid tumors ; Therapeutic targets ; Transcription factors ; Tumors ; Vincristine</subject><ispartof>Cell journal (Yakhteh), 2023-01, Vol.25 (1), p.1-10</ispartof><rights>2023. This work is published under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-582e5b629a8357144a55f974e47fd23b53efa6391bcc549dd67ccfa732a2c64c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2830850514/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2830850514?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25733,27903,27904,36991,36992,44569,53769,53771,74872</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36680478$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Asadi, Marjan</creatorcontrib><creatorcontrib>Gholampour, Mohammad Ali</creatorcontrib><creatorcontrib>Kompani, Farzad</creatorcontrib><creatorcontrib>Alizadeh, Shaban</creatorcontrib><title>Expression of Long Non-Coding RNA H19 in Acute Lymphoblastic Leukemia</title><title>Cell journal (Yakhteh)</title><addtitle>Cell J</addtitle><description>OObjective: Long non-coding RNA (lncRNA) H19 has essential roles in growth, migration, invasion, and metastasis of most cancers. H19 dysregulation is present in a large number of solid tumors and leukemia. However, the expression level of H19 in acute lymphoblastic leukemia (ALL) has not been elucidated yet. The current study aimed to explore
expression in ALL patients and cell lines.
This experimental study was conducted in bone marrow (BM) samples collected from 25 patients with newly diagnosed ALL. In addition, we cultured the RPMI-8402, Jurkat, Ramos, and Daudi cell lines and assessed the effects of internal (hypoxia) and external (chemotherapy medications L-asparaginase [ASP] and vincristine [VCR]) factors on h19 expression. The expressions of
and
were performed using quantitative real-time polymerase chain reaction (qRT-PCR) method.
There was significantly increased
expression in the B-cell ALL (B-ALL, P<0.05), T-cell ALL (T-ALL, P<0.01) patients and the cell lines. This upregulation was governed by the
and
transcription factors. We observed that increased
expression induced
expression; however,
adversely affected
expression. In addition, the results indicated that chemotherapy changed the gene expression pattern. There was a considerable decrease in
expression after exposure to chemotherapy medications; nonetheless, hypoxia induced
expression through
downregulation.
Our findings suggest that
may have an important role in pathogenesis in ALL and may act as a promising and potential therapeutic target.</description><subject>Actin</subject><subject>Acute lymphoblastic leukemia</subject><subject>Asparaginase</subject><subject>Bone marrow</subject><subject>c-Myc protein</subject><subject>Chemotherapy</subject><subject>Gene expression</subject><subject>h19</subject><subject>Hypoxia</subject><subject>Hypoxia-inducible factor 1a</subject><subject>L-asparaginase</subject><subject>Leukemia</subject><subject>lncrna</subject><subject>Lymphocytes B</subject><subject>Lymphocytes T</subject><subject>Metastases</subject><subject>Myc protein</subject><subject>Non-coding RNA</subject><subject>Original</subject><subject>p53 Protein</subject><subject>Pathogenesis</subject><subject>Polymerase chain reaction</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Solid tumors</subject><subject>Therapeutic targets</subject><subject>Transcription factors</subject><subject>Tumors</subject><subject>Vincristine</subject><issn>2228-5806</issn><issn>2228-5814</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkc1Lw0AQxRdRrKh3TxLw4qV1M_uZi1BKtYWgIHpeNptNuzXJ1mwi-t-7fqLOZYY3jx-PGYROUjwBwIJeGFvXmwlggIkkKdtBBwAgx0ymdPdnxnyEjkPY4FgcQ5rBPhoRziWmQh6g-fxl29kQnG8TXyW5b1fJjW_HM1-6ON7dTJNFmiWuTaZm6G2SvzbbtS9qHXpnktwOj7Zx-gjtVboO9virH6KHq_n9bDHOb6-Xs2k-NkTgPqYBywoOmZaEiZRSzViVCWqpqEogBSO20pxkaWEMo1lZcmFMpQUBDYZTQw7R8pNber1R2841untVXjv1IfhupXQXg9VWiQgtLFgumKSFNpJQLGURVZwJqUlkXX6ytkPR2NLYtu90_Qf6d9O6tVr5Z5VJLikREXD-Bej802BDrxoX3n-iW-uHoEBwCYRgzqL17J9144eujadSIAmWDLOURtfp70Q_Ub6_Rd4AQmKV_Q</recordid><startdate>20230101</startdate><enddate>20230101</enddate><creator>Asadi, Marjan</creator><creator>Gholampour, Mohammad Ali</creator><creator>Kompani, Farzad</creator><creator>Alizadeh, Shaban</creator><general>Royan Institute of Iran</general><general>Royan Institute</general><general>Royan Institute (ACECR), Tehran</general><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20230101</creationdate><title>Expression of Long Non-Coding RNA H19 in Acute Lymphoblastic Leukemia</title><author>Asadi, Marjan ; Gholampour, Mohammad Ali ; Kompani, Farzad ; Alizadeh, Shaban</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-582e5b629a8357144a55f974e47fd23b53efa6391bcc549dd67ccfa732a2c64c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Actin</topic><topic>Acute lymphoblastic leukemia</topic><topic>Asparaginase</topic><topic>Bone marrow</topic><topic>c-Myc protein</topic><topic>Chemotherapy</topic><topic>Gene expression</topic><topic>h19</topic><topic>Hypoxia</topic><topic>Hypoxia-inducible factor 1a</topic><topic>L-asparaginase</topic><topic>Leukemia</topic><topic>lncrna</topic><topic>Lymphocytes B</topic><topic>Lymphocytes T</topic><topic>Metastases</topic><topic>Myc protein</topic><topic>Non-coding RNA</topic><topic>Original</topic><topic>p53 Protein</topic><topic>Pathogenesis</topic><topic>Polymerase chain reaction</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Solid tumors</topic><topic>Therapeutic targets</topic><topic>Transcription factors</topic><topic>Tumors</topic><topic>Vincristine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Asadi, Marjan</creatorcontrib><creatorcontrib>Gholampour, Mohammad Ali</creatorcontrib><creatorcontrib>Kompani, Farzad</creatorcontrib><creatorcontrib>Alizadeh, Shaban</creatorcontrib><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Cell journal (Yakhteh)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Asadi, Marjan</au><au>Gholampour, Mohammad Ali</au><au>Kompani, Farzad</au><au>Alizadeh, Shaban</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Long Non-Coding RNA H19 in Acute Lymphoblastic Leukemia</atitle><jtitle>Cell journal (Yakhteh)</jtitle><addtitle>Cell J</addtitle><date>2023-01-01</date><risdate>2023</risdate><volume>25</volume><issue>1</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>2228-5806</issn><eissn>2228-5814</eissn><abstract>OObjective: Long non-coding RNA (lncRNA) H19 has essential roles in growth, migration, invasion, and metastasis of most cancers. H19 dysregulation is present in a large number of solid tumors and leukemia. However, the expression level of H19 in acute lymphoblastic leukemia (ALL) has not been elucidated yet. The current study aimed to explore
expression in ALL patients and cell lines.
This experimental study was conducted in bone marrow (BM) samples collected from 25 patients with newly diagnosed ALL. In addition, we cultured the RPMI-8402, Jurkat, Ramos, and Daudi cell lines and assessed the effects of internal (hypoxia) and external (chemotherapy medications L-asparaginase [ASP] and vincristine [VCR]) factors on h19 expression. The expressions of
and
were performed using quantitative real-time polymerase chain reaction (qRT-PCR) method.
There was significantly increased
expression in the B-cell ALL (B-ALL, P<0.05), T-cell ALL (T-ALL, P<0.01) patients and the cell lines. This upregulation was governed by the
and
transcription factors. We observed that increased
expression induced
expression; however,
adversely affected
expression. In addition, the results indicated that chemotherapy changed the gene expression pattern. There was a considerable decrease in
expression after exposure to chemotherapy medications; nonetheless, hypoxia induced
expression through
downregulation.
Our findings suggest that
may have an important role in pathogenesis in ALL and may act as a promising and potential therapeutic target.</abstract><cop>Iran</cop><pub>Royan Institute of Iran</pub><pmid>36680478</pmid><doi>10.22074/cellj.2022.8315</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Open Access: PubMed Central; Publicly Available Content Database |
| subjects | Actin Acute lymphoblastic leukemia Asparaginase Bone marrow c-Myc protein Chemotherapy Gene expression h19 Hypoxia Hypoxia-inducible factor 1a L-asparaginase Leukemia lncrna Lymphocytes B Lymphocytes T Metastases Myc protein Non-coding RNA Original p53 Protein Pathogenesis Polymerase chain reaction Ribonucleic acid RNA Solid tumors Therapeutic targets Transcription factors Tumors Vincristine |
| title | Expression of Long Non-Coding RNA H19 in Acute Lymphoblastic Leukemia |
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