Isolation and characterization of a motility-defective mutant of Euglena gracilis

• Published in: PeerJ (San Francisco, CA) CA), 2020-09, Vol.8, p.e10002-e10002, Article e10002
• Main Authors: Muramatsu, Shuki, Atsuji, Kohei, Yamada, Koji, Ozasa, Kazunari, Suzuki, Hideyuki, Takeuchi, Takuto, Hashimoto-Marukawa, Yuka, Kazama, Yusuke, Abe, Tomoko, Suzuki, Kengo, Iwata, Osamu
• Format: Article
• Language: English
• Subjects: Agricultural Science; Algae; Aquatic microorganisms; Biotechnology; Cell culture; Cell suspensions; Cellulose; Cultivars; Defective mutant; Euglena gracilis; Flagella; Genetics; Growth rate; Heavy-ion beam irradiation; Industrial applications; Ion beams; Light; Locomotion; Metabolism; Microalgae; Microbiology; Motility; Motility defect; Paramylon; Photosynthesis; Plant breeding; Population; Sedimentation; Swimming; β-1,3-Glucan
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.10002

is a green photosynthetic microalga that swims using its flagellum. This species has been used as a model organism for over half a century to study its metabolism and the mechanisms of its behavior. The development of mass-cultivation technology has led to application as a feedstock in various products such as foods. Therefore, breeding of has been attempted to improve the productivity of this feedstock for potential industrial applications. For this purpose, a characteristic that preserves the microalgal energy e.g., reduces motility, should be added to the cultivars. The objective of this study was to verify our hypothesis that locomotion-defective mutants are suitable for industrial applications because they save the energy required for locomotion. To test this hypothesis, we screened for mutants from Fe-ion-irradiated cell suspensions and established a mutant strain, ZFeL, which shows defects in flagellum formation and locomotion. The mutant strain exhibits a growth rate comparable to that of the wild type when cultured under autotrophic conditions, but had a slightly slower growth under heterotrophic conditions. It also stores 1.6 times the amount of paramylon, a crystal of -1,3-glucan, under autotrophic culture conditions, and shows a faster sedimentation compared with that of the wild type, because of the deficiency in mobility and probably the high amount of paramylon accumulation. Such characteristics make mutant cells suitable for cost-effective mass cultivation and harvesting.