RNA Pol II Assembly Affects ncRNA Expression

RNA pol II assembly occurs in the cytoplasm before translocation of the enzyme to the nucleus. Affecting this assembly influences mRNA transcription in the nucleus and mRNA decay in the cytoplasm. However, very little is known about the consequences on ncRNA synthesis. In this work, we show that imp...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-01, Vol.25 (1), p.507
Main Authors: Garrido-Godino, Ana I, Gupta, Ishaan, Pelechano, Vicent, Navarro, Francisco
Format: Article
Language:English
Subjects:
Cytoplasm
Enzymes
Genes
Genomes
Medicin och hälsovetenskap
Messenger RNA
MicroRNAs
Mutation
ncRNAs
NNS termination
Phosphatase
Phosphatases
Phosphorylation
RNA polymerases
RNA polymerases assembly
Rtr1 CTD phosphatase
transcription
Yeast
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Summary:RNA pol II assembly occurs in the cytoplasm before translocation of the enzyme to the nucleus. Affecting this assembly influences mRNA transcription in the nucleus and mRNA decay in the cytoplasm. However, very little is known about the consequences on ncRNA synthesis. In this work, we show that impairment of RNA pol II assembly leads to a decrease in cryptic non-coding RNAs (preferentially CUTs and SUTs). This alteration is partially restored upon overcoming the assembly defect. Notably, this drop in ncRNAs is only partially dependent on the nuclear exosome, which suggests a major specific effect of enzyme assembly. Our data also point out a defect in transcription termination, which leads us to propose that CTD phosphatase Rtr1 could be involved in this process.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25010507