Expression of Basophil Activation Markers in Pediatric Asthma

Asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms. To analyze the expression of ST2L and CD203c in the diagnosis of pediatric asthma. Basophils were purified from whole bloo...

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Bibliographic Details
Published in:Iranian journal of immunology 2016-03, Vol.13 (1), p.27-36
Main Authors: Abdullahi, Masooma, Ranjbaran, Reza, Alyasin, Soheaila, Keshavarz, Zeinab, Ramezani, Amin, Behzad-Behbahani, Abbas, Sharifzadeh, Sedigheh
Format: Article
Language:English
Subjects:
Airway management
Asthma
Asthma - genetics
Asthma - immunology
Basophil
Basophils - immunology
Basophils - metabolism
Biomarkers
Case-Control Studies
CD203c
Child, Preschool
Chronic illnesses
Cystic fibrosis
Diagnosis
Disease
Families & family life
Family medical history
Gene Expression
Genes
Humans
Infant
Infant, Newborn
Interleukin-1 Receptor-Like 1 Protein - genetics
Leukocytes (basophilic)
Mortality
Patients
Pediatrics
Phosphoric Diester Hydrolases - genetics
Pyrophosphatases - genetics
Real time
Respiratory diseases
ROC Curve
ST2L
Statistical analysis
Studies
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Summary:Asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms. To analyze the expression of ST2L and CD203c in the diagnosis of pediatric asthma. Basophils were purified from whole blood samples of patients and healthy controls using Ficol-Paque gradient and Basophil Isolation Kit. RNA extraction was done by RNX-Plus solution and after synthesis of cDNA, the gene expression was analyzed by means of real time PCR. Patients expressed significantly higher levels of CD203c than healthy controls (p=0.01). Although there was an increase in the transcription level of ST2L gene in patients, the results were not statistically significant compared to those obtained from the healthy controls (p>0.05). A Specificity of 60% and a sensitivity of 73% were found using ROC curve for CD203c expression. Patients with positive family history of asthma exhibited more CD203c and ST2L expression (p
ISSN:1735-1383
1735-367X