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Expression of Basophil Activation Markers in Pediatric Asthma
Asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms. To analyze the expression of ST2L and CD203c in the diagnosis of pediatric asthma. Basophils were purified from whole bloo...
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| Published in: | Iranian journal of immunology 2016-03, Vol.13 (1), p.27-36 |
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| container_end_page | 36 |
| container_issue | 1 |
| container_start_page | 27 |
| container_title | Iranian journal of immunology |
| container_volume | 13 |
| creator | Abdullahi, Masooma Ranjbaran, Reza Alyasin, Soheaila Keshavarz, Zeinab Ramezani, Amin Behzad-Behbahani, Abbas Sharifzadeh, Sedigheh |
| description | Asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms.
To analyze the expression of ST2L and CD203c in the diagnosis of pediatric asthma.
Basophils were purified from whole blood samples of patients and healthy controls using Ficol-Paque gradient and Basophil Isolation Kit. RNA extraction was done by RNX-Plus solution and after synthesis of cDNA, the gene expression was analyzed by means of real time PCR.
Patients expressed significantly higher levels of CD203c than healthy controls (p=0.01). Although there was an increase in the transcription level of ST2L gene in patients, the results were not statistically significant compared to those obtained from the healthy controls (p>0.05). A Specificity of 60% and a sensitivity of 73% were found using ROC curve for CD203c expression. Patients with positive family history of asthma exhibited more CD203c and ST2L expression (p |
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To analyze the expression of ST2L and CD203c in the diagnosis of pediatric asthma.
Basophils were purified from whole blood samples of patients and healthy controls using Ficol-Paque gradient and Basophil Isolation Kit. RNA extraction was done by RNX-Plus solution and after synthesis of cDNA, the gene expression was analyzed by means of real time PCR.
Patients expressed significantly higher levels of CD203c than healthy controls (p=0.01). Although there was an increase in the transcription level of ST2L gene in patients, the results were not statistically significant compared to those obtained from the healthy controls (p>0.05). A Specificity of 60% and a sensitivity of 73% were found using ROC curve for CD203c expression. Patients with positive family history of asthma exhibited more CD203c and ST2L expression (p<0.05).
It is proposed that determining CD203c expression by real time PCR may be an effective technique for diagnosis of pediatric asthma.</description><identifier>ISSN: 1735-1383</identifier><identifier>EISSN: 1735-367X</identifier><identifier>PMID: 27026044</identifier><language>eng</language><publisher>Iran: Shiraz Institute for Cancer Research</publisher><subject>Airway management ; Asthma ; Asthma - genetics ; Asthma - immunology ; Basophil ; Basophils - immunology ; Basophils - metabolism ; Biomarkers ; Case-Control Studies ; CD203c ; Child, Preschool ; Chronic illnesses ; Cystic fibrosis ; Diagnosis ; Disease ; Families & family life ; Family medical history ; Gene Expression ; Genes ; Humans ; Infant ; Infant, Newborn ; Interleukin-1 Receptor-Like 1 Protein - genetics ; Leukocytes (basophilic) ; Mortality ; Patients ; Pediatrics ; Phosphoric Diester Hydrolases - genetics ; Pyrophosphatases - genetics ; Real time ; Respiratory diseases ; ROC Curve ; ST2L ; Statistical analysis ; Studies</subject><ispartof>Iranian journal of immunology, 2016-03, Vol.13 (1), p.27-36</ispartof><rights>Copyright Shiraz Institute for Cancer Research Mar 2016</rights><rights>Copyright Shiraz Institute for Cancer Research 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27026044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abdullahi, Masooma</creatorcontrib><creatorcontrib>Ranjbaran, Reza</creatorcontrib><creatorcontrib>Alyasin, Soheaila</creatorcontrib><creatorcontrib>Keshavarz, Zeinab</creatorcontrib><creatorcontrib>Ramezani, Amin</creatorcontrib><creatorcontrib>Behzad-Behbahani, Abbas</creatorcontrib><creatorcontrib>Sharifzadeh, Sedigheh</creatorcontrib><title>Expression of Basophil Activation Markers in Pediatric Asthma</title><title>Iranian journal of immunology</title><addtitle>Iran J Immunol</addtitle><description>Asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms.
To analyze the expression of ST2L and CD203c in the diagnosis of pediatric asthma.
Basophils were purified from whole blood samples of patients and healthy controls using Ficol-Paque gradient and Basophil Isolation Kit. RNA extraction was done by RNX-Plus solution and after synthesis of cDNA, the gene expression was analyzed by means of real time PCR.
Patients expressed significantly higher levels of CD203c than healthy controls (p=0.01). Although there was an increase in the transcription level of ST2L gene in patients, the results were not statistically significant compared to those obtained from the healthy controls (p>0.05). A Specificity of 60% and a sensitivity of 73% were found using ROC curve for CD203c expression. Patients with positive family history of asthma exhibited more CD203c and ST2L expression (p<0.05).
It is proposed that determining CD203c expression by real time PCR may be an effective technique for diagnosis of pediatric asthma.</description><subject>Airway management</subject><subject>Asthma</subject><subject>Asthma - genetics</subject><subject>Asthma - immunology</subject><subject>Basophil</subject><subject>Basophils - immunology</subject><subject>Basophils - metabolism</subject><subject>Biomarkers</subject><subject>Case-Control Studies</subject><subject>CD203c</subject><subject>Child, Preschool</subject><subject>Chronic illnesses</subject><subject>Cystic fibrosis</subject><subject>Diagnosis</subject><subject>Disease</subject><subject>Families & family life</subject><subject>Family medical history</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Interleukin-1 Receptor-Like 1 Protein - genetics</subject><subject>Leukocytes (basophilic)</subject><subject>Mortality</subject><subject>Patients</subject><subject>Pediatrics</subject><subject>Phosphoric Diester Hydrolases - genetics</subject><subject>Pyrophosphatases - genetics</subject><subject>Real time</subject><subject>Respiratory diseases</subject><subject>ROC Curve</subject><subject>ST2L</subject><subject>Statistical analysis</subject><subject>Studies</subject><issn>1735-1383</issn><issn>1735-367X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNqNkU1Lw0AQhoMotlb_ggS8eAnsV3aTg4daqhYqelDwFib7Ybcm2bibiP57U1s9eBBPMzw8vPDO7EVjLGiaUC6e9nc7phkdRUchrBHiHGF0GI2IQIQjxsbRxfy99ToE65rYmfgSgmtXtoqnsrNv0G3wLfgX7UNsm_heKwudtzKehm5Vw3F0YKAK-mQ3J9Hj1fxhdpMs764Xs-kyUZTzLgFjkObGgJAlZgxTgYkEpHipiMmkQUB0jlMFCpOBASVE5WjgOSjJQNNJtNjmKgfrovW2Bv9ROLDFF3D-uQDfWVnpQuhUGKp5KkjJiElLSYVgnKYm45gZPGSdb7Na7157HbqitkHqqoJGuz4UWOQoZwKn7B_qEJ1lPKWDevZLXbveN8NRCpKxzeEFEn9ZWGSZYHSoPlinO6sva61--n5_jX4COjuRhg</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Abdullahi, Masooma</creator><creator>Ranjbaran, Reza</creator><creator>Alyasin, Soheaila</creator><creator>Keshavarz, Zeinab</creator><creator>Ramezani, Amin</creator><creator>Behzad-Behbahani, Abbas</creator><creator>Sharifzadeh, Sedigheh</creator><general>Shiraz Institute for Cancer Research</general><general>Shiraz University of Medical Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20160301</creationdate><title>Expression of Basophil Activation Markers in Pediatric Asthma</title><author>Abdullahi, Masooma ; Ranjbaran, Reza ; Alyasin, Soheaila ; Keshavarz, Zeinab ; Ramezani, Amin ; Behzad-Behbahani, Abbas ; Sharifzadeh, Sedigheh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d366t-aff0e6ffa7cb14413712ca0d6bd2f8cf0a2e915dad126bda322d90cf09adc4ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Airway management</topic><topic>Asthma</topic><topic>Asthma - genetics</topic><topic>Asthma - immunology</topic><topic>Basophil</topic><topic>Basophils - immunology</topic><topic>Basophils - metabolism</topic><topic>Biomarkers</topic><topic>Case-Control Studies</topic><topic>CD203c</topic><topic>Child, Preschool</topic><topic>Chronic illnesses</topic><topic>Cystic fibrosis</topic><topic>Diagnosis</topic><topic>Disease</topic><topic>Families & family life</topic><topic>Family medical history</topic><topic>Gene Expression</topic><topic>Genes</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Interleukin-1 Receptor-Like 1 Protein - genetics</topic><topic>Leukocytes (basophilic)</topic><topic>Mortality</topic><topic>Patients</topic><topic>Pediatrics</topic><topic>Phosphoric Diester Hydrolases - genetics</topic><topic>Pyrophosphatases - genetics</topic><topic>Real time</topic><topic>Respiratory diseases</topic><topic>ROC Curve</topic><topic>ST2L</topic><topic>Statistical analysis</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abdullahi, Masooma</creatorcontrib><creatorcontrib>Ranjbaran, Reza</creatorcontrib><creatorcontrib>Alyasin, Soheaila</creatorcontrib><creatorcontrib>Keshavarz, Zeinab</creatorcontrib><creatorcontrib>Ramezani, Amin</creatorcontrib><creatorcontrib>Behzad-Behbahani, Abbas</creatorcontrib><creatorcontrib>Sharifzadeh, Sedigheh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Iranian journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abdullahi, Masooma</au><au>Ranjbaran, Reza</au><au>Alyasin, Soheaila</au><au>Keshavarz, Zeinab</au><au>Ramezani, Amin</au><au>Behzad-Behbahani, Abbas</au><au>Sharifzadeh, Sedigheh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Basophil Activation Markers in Pediatric Asthma</atitle><jtitle>Iranian journal of immunology</jtitle><addtitle>Iran J Immunol</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>13</volume><issue>1</issue><spage>27</spage><epage>36</epage><pages>27-36</pages><issn>1735-1383</issn><eissn>1735-367X</eissn><abstract>Asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms.
To analyze the expression of ST2L and CD203c in the diagnosis of pediatric asthma.
Basophils were purified from whole blood samples of patients and healthy controls using Ficol-Paque gradient and Basophil Isolation Kit. RNA extraction was done by RNX-Plus solution and after synthesis of cDNA, the gene expression was analyzed by means of real time PCR.
Patients expressed significantly higher levels of CD203c than healthy controls (p=0.01). Although there was an increase in the transcription level of ST2L gene in patients, the results were not statistically significant compared to those obtained from the healthy controls (p>0.05). A Specificity of 60% and a sensitivity of 73% were found using ROC curve for CD203c expression. Patients with positive family history of asthma exhibited more CD203c and ST2L expression (p<0.05).
It is proposed that determining CD203c expression by real time PCR may be an effective technique for diagnosis of pediatric asthma.</abstract><cop>Iran</cop><pub>Shiraz Institute for Cancer Research</pub><pmid>27026044</pmid><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Freely Accessible Science Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Airway management Asthma Asthma - genetics Asthma - immunology Basophil Basophils - immunology Basophils - metabolism Biomarkers Case-Control Studies CD203c Child, Preschool Chronic illnesses Cystic fibrosis Diagnosis Disease Families & family life Family medical history Gene Expression Genes Humans Infant Infant, Newborn Interleukin-1 Receptor-Like 1 Protein - genetics Leukocytes (basophilic) Mortality Patients Pediatrics Phosphoric Diester Hydrolases - genetics Pyrophosphatases - genetics Real time Respiratory diseases ROC Curve ST2L Statistical analysis Studies |
| title | Expression of Basophil Activation Markers in Pediatric Asthma |
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