D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis

We aimed to compare the time-course effect of D-galactose (D-gal)-induced aging, obesity, and their combined effects on bone homeostasis. Male Wistar rats were fed with either a normal diet (ND; n = 24) or a high-fat diet (HFD; n = 24) for 12 weeks. All rats were then injected with either vehicle or...

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Bibliographic Details
Published in:Scientific reports 2022-05, Vol.12 (1), p.8580-8580, Article 8580
Main Authors: Imerb, Napatsorn, Thonusin, Chanisa, Pratchayasakul, Wasana, Arunsak, Busarin, Nawara, Wichwara, Ongnok, Benjamin, Aeimlapa, Ratchaneevan, Charoenphandhu, Narattaphol, Chattipakorn, Nipon, Chattipakorn, Siriporn C.
Format: Article
Language:English
Subjects:
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Advanced glycosylation end products
Aging
Bone histomorphometry
Bone resorption
Bone turnover
D-Galactose
Galactose
Glycosylation
High fat diet
Homeostasis
Humanities and Social Sciences
Inflammation
Insulin
multidisciplinary
Obesity
Osteoclastogenesis
Oxidative stress
Rodents
Science
Science (multidisciplinary)
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Summary:We aimed to compare the time-course effect of D-galactose (D-gal)-induced aging, obesity, and their combined effects on bone homeostasis. Male Wistar rats were fed with either a normal diet (ND; n = 24) or a high-fat diet (HFD; n = 24) for 12 weeks. All rats were then injected with either vehicle or 150 mg/kg/day of D-gal for 4 or 8 weeks. Blood was collected to measure metabolic, aging, oxidative stress, and bone turnover parameters. Bone oxidative stress and inflammatory markers, as well as bone histomorphometry were also evaluated. Additionally, RAW 264.7 cells were incubated with either D-gal, insulin, or D-gal plus insulin to identify osteoclast differentiation capacity under the stimulation of receptor activator of nuclear factor κB ligand. At week 4, D-gal-induced aging significantly elevated serum malondialdehyde level and decreased trabecular thickness in ND- and HFD-fed rats, when compared to the control group. At week 8, D-gal-induced aging further elevated advanced glycation end products, increased bone inflammation and resorption, and significantly impaired bone microarchitecture in HFD-fed rats. The osteoclast number in vitro were increased in the D-gal, insulin, and combined groups to a similar extent. These findings suggest that aging aggravates bone dyshomeostasis in the obese condition in a time-dependent manner.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-12206-4