Intestinal microbiota and metabolome perturbations in ischemic and idiopathic dilated cardiomyopathy

• Published in: Journal of translational medicine 2024-01, Vol.22 (1), p.89-19, Article 89
• Main Authors: Wang, Yusheng, Xie, Yandan, Mahara, Gehendra, Xiong, Yanling, Xiong, Yalan, Zheng, Qifang, Chen, Jianqin, Zhang, Wei, Zhou, Honghao, Li, Qing
• Format: Article
• Language: English
• Subjects: Analysis; Biological diversity; Cardiomyopathy; Cardiomyopathy, Dilated; Cardiovascular disease; Care and treatment; Community structure; Composition; Coronary vessels; Diagnosis; Digestive system; Dilated cardiomyopathy; Dysbacteriosis; Dysbiosis; Ejection fraction; Feces; Gas chromatography; Gastrointestinal Microbiome; Gastrointestinal tract; Heart failure; Humans; Hypertension; Idiopathic dilated cardiomyopathy; Intestinal microflora; Intestine; Investigations; Ischemia; Ischemic dilated cardiomyopathy; Mass spectroscopy; Medical imaging; Metabolites; Metabolome; Metabolomics; Microbiota; Microbiota (Symbiotic organisms); Multi-omics analysis; Physiology; RNA, Ribosomal, 16S - genetics; rRNA 16S; Software; Taxonomy; Vein & artery diseases
• ISSN: 1479-5876; 1479-5876
• DOI: 10.1186/s12967-023-04605-6

Various clinical similarities are present in ischemic (ICM) and idiopathic dilated cardiomyopathy (IDCM), leading to ambiguity on some occasions. Previous studies have reported that intestinal microbiota appeared dysbiosis in ICM, whether implicating in the IDCM remains unclear. The aim of this study was to assess the alterations in intestinal microbiota and fecal metabolites in ICM and IDCM. ICM (n = 20), IDCM (n = 22), and healthy controls (HC, n = 20) were enrolled in this study. Stool samples were collected for 16S rRNA gene sequencing and gas chromatography-mass spectrometry (GC-MS) analysis. Both ICM and IDCM exhibited reduced alpha diversity and altered microbial community structure compared to HC. At the genus level, nine taxa including Blautia, [Ruminococcus]_torques_group, Christensenellaceae_R-7_group, UCG-002, Corynebacterium, Oceanobacillus, Gracilibacillus, Klebsiella and Citrobacter was specific to ICM, whereas one taxa Alistipes uniquely altered in IDCM. Likewise, these changes were accompanied by significant metabolic differences. Further differential analysis displayed that 18 and 14 specific metabolites uniquely changed in ICM and IDCM, respectively. The heatmap was generated to display the association between genera and metabolites. Receiver operating characteristic curve (ROC) analysis confirmed the predictive value of the distinct microbial-metabolite features in disease status. The results showed that microbial (area under curve, AUC = 0.95) and metabolic signatures (AUC = 0.84) were effective in discriminating ICM from HC. Based on the specific microbial and metabolic features, the patients with IDCM could be separated from HC with an AUC of 0.80 and 0.87, respectively. Furthermore, the gut microbial genus (AUC = 0.88) and metabolite model (AUC = 0.89) were comparable in predicting IDCM from ICM. Especially, the combination of fecal microbial-metabolic features improved the ability to differentiate IDCM from ICM with an AUC of 0.96. Our findings highlighted the alterations of gut microbiota and metabolites in different types of cardiomyopathies, providing insights into the pathophysiological mechanisms of myocardial diseases. Moreover, multi-omics analysis of fecal samples holds promise as a non-invasive tool for distinguishing disease status.