Characterization of bone repair in rat femur after treatment with calcium phosphate cement and autogenous bone graft

• Published in: Head & face medicine 2010-06, Vol.6 (1), p.10-10, Article 10
• Main Authors: Puricelli, Edela, Corsetti, Adriana, Ponzoni, Deise, Martins, Gustavo L, Leite, Mauro G, Santos, Luis A
• Format: Article
• Language: English
• Subjects: Animals; Biocompatibility; Bone Cements; Bone regeneration; Bone Substitutes; Bone Transplantation; Calcium phosphate; Calcium phosphates; Calcium Phosphates - therapeutic use; Cement; Cementation - methods; Chemical properties; Dentistry; Disease Models, Animal; Femur - transplantation; Health aspects; Male; Medical research; Rats; Rats, Wistar; Studies; Transplantation, Autologous
• ISSN: 1746-160X; 1746-160X
• DOI: 10.1186/1746-160X-6-10

In this study, the biocompatibility, stability and osteotransductivity of a new cement based on alpha-tricalcium phosphate (alpha-TCP) were investigated in a bone repair model using a rat model. The potential of alpha-TCP on bone repair was compared to autogenous bone grafting, and unfilled cavities were used as negative control. Surgical cavities were prepared and designated as test (T), implanted with alpha-TCP blocks; negative control (C - ), unfilled; and positive control (C + ), implanted with autogenous bone graft. Results were analyzed on postoperative days three, seven, 14, 21 and 60. The histological analyses showed the following results. Postoperative day three: presence of inflammatory infiltrate, erythrocytes and proliferating fibroblasts in T, C - and C + samples. Day seven: extensive bone neoformation in groups T and C + , and beginning of alpha-TCP resorption by phagocytic cells. Days 14 and 21: osteoblastic activity in the three types of cavities. Day 60: In all samples, neoformed bone similar to surrounding bone. Moderate interruption on the ostectomized cortical bone. Bone neoformation is seen seven days after implantation of alpha-TCP and autogenous bone. Comparison of C - with T and C + samples showed that repair is faster in implanted cavities; on day 60, control groups presented almost complete bone repair. Alpha-TCP cement presents biocompatibility and osteotransductivity, besides stability, but 60 days after surgery the cavities were not closed.