Freeze-drying of Beauveria bassiana suspended in Hydroxyethyl cellulose based hydrogel as possible method for storage: Evaluation of survival, growth and stability of conidial concentration before and after processing

Beauveria bassiana (Bb) is an entomopathogenic fungus considered as a valid alternative to chemical pesticides. However, Bb use is still limited due to short storage period and persistence in field conditions. Storage can be extended by reducing water content in products but an excessive drying can...

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Bibliographic Details
Published in:Results in engineering 2021-12, Vol.12, p.100283, Article 100283
Main Authors: Friuli, Marco, Nitti, Paola, Aneke, Chioma I., Demitri, Christian, Cafarchia, Claudia, Otranto, Domenico
Format: Article
Language:English
Subjects:
Beauveria bassiana
Hydrogel
Hydroxyethyl cellulose
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Summary:Beauveria bassiana (Bb) is an entomopathogenic fungus considered as a valid alternative to chemical pesticides. However, Bb use is still limited due to short storage period and persistence in field conditions. Storage can be extended by reducing water content in products but an excessive drying can cause damage during rehydration. Persistence is promoted by using conidia into pellets or granules formulation. Integration of these two aspects can be challenging and costly. In this work, we present how utilizing a hydrogel containing Bb as growth substrate could potentially overcome both issues. •Beauveria bassiana/gel systems were obtained by adding HEC to conidial suspensions.•HEC extended the vitality and growth of Beauveria bassiana (Bb) up to 24 days.•Bb/Gel vitality and growth was restored after freeze-drying and rehydration.•Bb freeze drying into HEC can reduce storage costs.
ISSN:2590-1230
2590-1230
DOI:10.1016/j.rineng.2021.100283