Propagation of Inoculum for Haematococcus pluvialis Microalgae Scale-Up Photobioreactor Cultivation System

An increasing number of microalgae strains are used for commercial production of metabolites. When conducting research, the moment of the process scaling tends to be very difficult. One of the most complex issues is related to planning and designing an efficient system for propagation of appropriate...

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Bibliographic Details
Published in:Applied sciences 2020-09, Vol.10 (18), p.6283
Main Authors: Borowiak, Daniel, Pstrowska, Katarzyna, Wiśniewski, Maciej, Grzebyk, Michał
Format: Article
Language:English
Subjects:
Algae
Biomass
bioprocess control
Control systems
Cultivation
Ergonomics
Experiments
Flowers & plants
Haematococcus pluvialis
Inoculation
Inoculum
Laboratories
Metabolites
microalgae
Microorganisms
photobioreactor
Photobioreactors
Propagation
R&D
Research & development
scaling-up
Solar energy
Storage units
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Summary:An increasing number of microalgae strains are used for commercial production of metabolites. When conducting research, the moment of the process scaling tends to be very difficult. One of the most complex issues is related to planning and designing an efficient system for propagation of appropriately high amounts of inoculum required for inoculating cultures on a semi-technical and industrial scale. The following paper aimed at designing an automated station for the preparation of microalgae inoculation material intended for inoculation of the system, comprising of six 90 dm3 volume photobioreactors. The system, comprised of eight airlift photobioreactors of 12 dm3 volume each, installed in mobile storage units connected to the control system in the form of a docking station. Each of the photobioreactors had a separate system used for monitoring temperature and pH, mixing, and LED lighting. The station constituted the last stage of preparing the inoculation material for inoculating technical-scale photobioreactors, used for conducting experiments with Haematococcus pluvialis microalgae. Achieved results, repeatability of the processes, and the ergonomics of the station increased the productivity and quality of the research and development processes.
ISSN:2076-3417
2076-3417
DOI:10.3390/app10186283