In Vivo Simultaneous Analysis of Gene Expression by Dual-Color Luciferases in Caenorhabditis elegans

Both fluorescent and luminescent observation are widely used to examine real-time gene expression patterns in living organisms. Several fluuorescent and luminescent proteins with specific optical properties have been developed and applied for simultaneous, multi-color observation of more than two ge...

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Bibliographic Details
Published in:International journal of molecular sciences 2020-12, Vol.22 (1), p.119
Main Authors: Doi, Motomichi, Sato, Megumi, Ohmiya, Yoshihiro
Format: Article
Language:English
Subjects:
Animals
Beryl
bioluminescence
C. elegans
Embryos
Environmental conditions
Fluorescence
Gene expression
Genes
Life span
multi-color luciferases
Nematodes
Optical properties
Proteins
Worms
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Summary:Both fluorescent and luminescent observation are widely used to examine real-time gene expression patterns in living organisms. Several fluuorescent and luminescent proteins with specific optical properties have been developed and applied for simultaneous, multi-color observation of more than two gene expression profiles. Compared to fluorescent proteins, however, the application of multi-color luminescent imaging in living organisms is still limited. In this study, we introduced two-color luciferases into the soil nematode and performed simultaneous analysis of two gene expression profiles. Using a green-emitting luciferase Eluc (emerald luciferase) and red-emitting luciferase SLR (stable luciferase red), the expression patterns of two genes were simultaneously observed in single animals from embryonic to adult stages over its whole life span. In addition, dual gene activities were observed at the single embryo level, with the simultaneous observation of morphological changes. These are the first application of a two-color luciferase system into a whole animal and suggest that precise relationship of expression patterns of multiple genes of interest can be analyzed over the whole life of the animal, dependent on the changes in genetic and/or environmental conditions.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22010119