Differential Expression and Bioinformatics Analysis of Plasma-Derived Exosomal circRNA in Type 1 Diabetes Mellitus

Backgrounds. Both exosome and circular RNA (circRNA) have been reported to participate in the pathogenesis of type 1 diabetes mellitus (T1DM). However, the exact role of exosomal circRNA in T1DM is largely unknown. Here, we identified the exosomal circRNA expression profiles in the plasma of T1DM pa...

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Bibliographic Details
Published in:Journal of immunology research 2022-10, Vol.2022, p.1-10
Main Authors: Pang, Haipeng, Fan, Wenqi, Shi, Xiajie, Luo, Shuoming, Wang, Yimeng, Lin, Jian, Xiao, Yang, Li, Xia, Huang, Gan, Xie, Zhiguo, Zhou, Zhiguang
Format: Article
Language:English
Subjects:
Bioinformatics
Biomarkers
Circular RNA
Diabetes
Diabetes mellitus (insulin dependent)
Exosomes
Gene expression
Genomes
Immunology
Membrane proteins
miRNA
Molecular modelling
mRNA
Plasma
Proteolysis
Software
Stem cells
Ubiquitin
Ubiquitination
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Summary:Backgrounds. Both exosome and circular RNA (circRNA) have been reported to participate in the pathogenesis of type 1 diabetes mellitus (T1DM). However, the exact role of exosomal circRNA in T1DM is largely unknown. Here, we identified the exosomal circRNA expression profiles in the plasma of T1DM patients and explored their potential function using bioinformatics analysis. Material and Methods. Exosomes were extracted by the size exclusion chromatography method from plasma of 10 T1DM patients and 10 age- and sex- matched control subjects. Illumina Novaseq6000 platform was used to detect the exosomal circRNA expression profiles. Multiple bioinformatics analysis was applied to investigate the potential biological functions of exosomal circRNAs. Results. A total of 784 differentially expressed exosomal circRNAs have been identified in T1DM patients, of which 528 were upregulated and 256 were downregulated. Gene Ontology analysis enriched terms such as protein ubiquitination involved in ubiquitin-dependent protein catabolic protein (GO:0042787), membrane (GO:0016020), and GTPase activator activity (GO:0005096). The most enriched pathway in Kyoto Encyclopedia of Genes and Genomes was ubiquitin-mediated proteolysis (ko04120). The miRNA-targeting prediction method was used to identify the miRNAs that bind to circRNAs, and circRNA-miRNA-mRNA pathways were constructed, indicating that interactions between circRNA, miRNA, and gene might be involved in the disease progression. Conclusions. The present study identified the exosomal circRNA expression profiles in T1DM for the first time. Our results threw novel insights into the molecular mechanisms of T1DM.
ISSN:2314-8861
2314-7156
DOI:10.1155/2022/3625052