Monocyte biology conserved across species: Functional insights from cattle

Similar to human monocytes, bovine monocytes can be split into CD14 CD16 classical, CD14 CD16 intermediate and CD14 CD16 nonclassical monocytes (cM, intM, and ncM, respectively). Here, we present an in-depth analysis of their steady-state bulk- and single-cell transcriptomes, highlighting both prono...

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Bibliographic Details
Published in:Frontiers in immunology 2022-07, Vol.13, p.889175-889175
Main Authors: Talker, Stephanie C, Barut, G Tuba, Lischer, Heidi E L, Rufener, Reto, von Münchow, Lilly, Bruggmann, Rémy, Summerfield, Artur
Format: Article
Language:English
Subjects:
Animals
Antigen Presentation
Antiviral Agents - metabolism
Biology
bovine monocyte subsets
Cattle
Cell Differentiation
classical monocytes
Humans
Immunology
intermediate monocytes
Monocytes
nonclassical monocytes
single-cell RNA sequencing (scRNA-seq)
transcriptome
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Summary:Similar to human monocytes, bovine monocytes can be split into CD14 CD16 classical, CD14 CD16 intermediate and CD14 CD16 nonclassical monocytes (cM, intM, and ncM, respectively). Here, we present an in-depth analysis of their steady-state bulk- and single-cell transcriptomes, highlighting both pronounced functional specializations and transcriptomic relatedness. Bulk gene transcription indicates pro-inflammatory and antibacterial roles of cM, while ncM and intM appear to be specialized in regulatory/anti-inflammatory functions and tissue repair, as well as antiviral responses and T-cell immunomodulation. Notably, intM stood out by high expression of several genes associated with antigen presentation. Anti-inflammatory and antiviral functions of ncM are further supported by dominant oxidative phosphorylation and selective strong responses to TLR7/8 ligands, respectively. Moreover, single-cell RNA-seq revealed previously unappreciated heterogeneity within cM and proposes intM as a transient differentiation intermediate between cM and ncM.
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2022.889175