Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates

Senescence is a natural phenomenon of growing old. It accelerates under certain conditions like diabetes mellitus resulting in early decline of bodily functions, which can be avoided by many claimed functional foods. The present study aims to investigate the anti-aging ability of Fenugreek seeds (Tr...

Full description

Saved in:
Bibliographic Details
Published in:Heliyon 2023-06, Vol.9 (6), p.e16866-e16866, Article e16866
Main Authors: Anwar, Laila, Ali, Syed Abid, Khan, Sana, Uzairullah, Mir Muhammad, Mustafa, Nazish, Ali, Urooj Anwer, Siddiqui, Faheema, Bhatti, Huma Aslam, Rehmani, Syed Jawad, Abbas, Ghulam
Format: Article
Language:English
Subjects:
Anti-aging functional foods
Carbonyl trapping
Glycation
Trigonellafoenum-graecum
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Senescence is a natural phenomenon of growing old. It accelerates under certain conditions like diabetes mellitus resulting in early decline of bodily functions, which can be avoided by many claimed functional foods. The present study aims to investigate the anti-aging ability of Fenugreek seeds (Trigonellafoenum-graecum); a common ingredient of Indo-Pak cuisines. Briefly, the Fenugreek seeds extract (FgSE) in concentrationsof0.1, 0.5 and 1 mg/ml inhibited the formation of Advanced Glycation End products (AGEs) and fructosamine adducts in Bovine serum albumin (BSA)/fructose model in vitro. The BSA conformational analysis via Circular Dichorism and Congo red assays showed that it preserves secondary structure of BSA in aforementioned model. Although mechanistic studies revealed insignificant lysine blocking ability of Fenugreek by OPA assay, however carbonyl entrapping was found to be 24%, 34% and 42% at 0.1, 0.5 and 1 mg/ml, respectively. In vivo model of High Fructose diet (HFD) induced glycation, FgSE treatment in doses of 10, 25 & 50 mg/kg markedly improved Escape latency (p 
ISSN:2405-8440
2405-8440
DOI:10.1016/j.heliyon.2023.e16866