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Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates
Senescence is a natural phenomenon of growing old. It accelerates under certain conditions like diabetes mellitus resulting in early decline of bodily functions, which can be avoided by many claimed functional foods. The present study aims to investigate the anti-aging ability of Fenugreek seeds (Tr...
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Published in: | Heliyon 2023-06, Vol.9 (6), p.e16866-e16866, Article e16866 |
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creator | Anwar, Laila Ali, Syed Abid Khan, Sana Uzairullah, Mir Muhammad Mustafa, Nazish Ali, Urooj Anwer Siddiqui, Faheema Bhatti, Huma Aslam Rehmani, Syed Jawad Abbas, Ghulam |
description | Senescence is a natural phenomenon of growing old. It accelerates under certain conditions like diabetes mellitus resulting in early decline of bodily functions, which can be avoided by many claimed functional foods. The present study aims to investigate the anti-aging ability of Fenugreek seeds (Trigonellafoenum-graecum); a common ingredient of Indo-Pak cuisines. Briefly, the Fenugreek seeds extract (FgSE) in concentrationsof0.1, 0.5 and 1 mg/ml inhibited the formation of Advanced Glycation End products (AGEs) and fructosamine adducts in Bovine serum albumin (BSA)/fructose model in vitro. The BSA conformational analysis via Circular Dichorism and Congo red assays showed that it preserves secondary structure of BSA in aforementioned model. Although mechanistic studies revealed insignificant lysine blocking ability of Fenugreek by OPA assay, however carbonyl entrapping was found to be 24%, 34% and 42% at 0.1, 0.5 and 1 mg/ml, respectively. In vivo model of High Fructose diet (HFD) induced glycation, FgSE treatment in doses of 10, 25 & 50 mg/kg markedly improved Escape latency (p |
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•Fenugreek seeds extract (FgSE) inhibited formation of advanced glycation end products.•Fenugreek seeds extract preserved the secondary structure of BSA in the presence of fructose.•Fenugreek seeds extract entrap the reactive carbonyl compounds required for the synthesis of AGEs.•Fenugreek seeds extract prevented the glycation-induced cognitive decline.•Fenugreek seeds extract reduced the CML levels in both hippocampus and serum of high fructose fed rats.</description><identifier>ISSN: 2405-8440</identifier><identifier>EISSN: 2405-8440</identifier><identifier>DOI: 10.1016/j.heliyon.2023.e16866</identifier><identifier>PMID: 37484294</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Anti-aging functional foods ; Carbonyl trapping ; Glycation ; Trigonellafoenum-graecum</subject><ispartof>Heliyon, 2023-06, Vol.9 (6), p.e16866-e16866, Article e16866</ispartof><rights>2023 The Authors</rights><rights>2023 The Authors.</rights><rights>2023 The Authors 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c534t-c390964c37dac6e183ac0f8a75d402a30c3b4fd487fcead419280dc844770a523</citedby><cites>FETCH-LOGICAL-c534t-c390964c37dac6e183ac0f8a75d402a30c3b4fd487fcead419280dc844770a523</cites><orcidid>0000-0002-4774-2728 ; 0000-0001-6559-766X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10360956/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S2405844023040732$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3549,27924,27925,45780,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37484294$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anwar, Laila</creatorcontrib><creatorcontrib>Ali, Syed Abid</creatorcontrib><creatorcontrib>Khan, Sana</creatorcontrib><creatorcontrib>Uzairullah, Mir Muhammad</creatorcontrib><creatorcontrib>Mustafa, Nazish</creatorcontrib><creatorcontrib>Ali, Urooj Anwer</creatorcontrib><creatorcontrib>Siddiqui, Faheema</creatorcontrib><creatorcontrib>Bhatti, Huma Aslam</creatorcontrib><creatorcontrib>Rehmani, Syed Jawad</creatorcontrib><creatorcontrib>Abbas, Ghulam</creatorcontrib><title>Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates</title><title>Heliyon</title><addtitle>Heliyon</addtitle><description>Senescence is a natural phenomenon of growing old. It accelerates under certain conditions like diabetes mellitus resulting in early decline of bodily functions, which can be avoided by many claimed functional foods. The present study aims to investigate the anti-aging ability of Fenugreek seeds (Trigonellafoenum-graecum); a common ingredient of Indo-Pak cuisines. Briefly, the Fenugreek seeds extract (FgSE) in concentrationsof0.1, 0.5 and 1 mg/ml inhibited the formation of Advanced Glycation End products (AGEs) and fructosamine adducts in Bovine serum albumin (BSA)/fructose model in vitro. The BSA conformational analysis via Circular Dichorism and Congo red assays showed that it preserves secondary structure of BSA in aforementioned model. Although mechanistic studies revealed insignificant lysine blocking ability of Fenugreek by OPA assay, however carbonyl entrapping was found to be 24%, 34% and 42% at 0.1, 0.5 and 1 mg/ml, respectively. In vivo model of High Fructose diet (HFD) induced glycation, FgSE treatment in doses of 10, 25 & 50 mg/kg markedly improved Escape latency (p < 0.01) and preserved cognition in Morris Water Maze. Our data further exhibits significant decrease of CML (Nε-carboxymethyl lysine) levels in serum and hippocampus byFgSE treatment in comparison with HFD group. Therefore, we deduced that FgSE prevents glycation-induced memory decline via entrapping the reactive carbonyl intermediates, formed during production of AGEs. Hence, as a promising functional food it slows down the harmful process of glycation and aging associated morbidities.
•Fenugreek seeds extract (FgSE) inhibited formation of advanced glycation end products.•Fenugreek seeds extract preserved the secondary structure of BSA in the presence of fructose.•Fenugreek seeds extract entrap the reactive carbonyl compounds required for the synthesis of AGEs.•Fenugreek seeds extract prevented the glycation-induced cognitive decline.•Fenugreek seeds extract reduced the CML levels in both hippocampus and serum of high fructose fed rats.</description><subject>Anti-aging functional foods</subject><subject>Carbonyl trapping</subject><subject>Glycation</subject><subject>Trigonellafoenum-graecum</subject><issn>2405-8440</issn><issn>2405-8440</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNqFks9uEzEQxlcIRKvSRwD5yCVhvPZ6d08IVRQqVeICZ8sZz24cNnawnRV5AZ4bh4TSnjjZmj-_8Xz-quo1hyUHrt5tlmua3CH4ZQ21WBJXnVLPqstaQrPopITnj-4X1XVKGwDgTaf6VrysLkQrO1n38rL6dUt-P0ai7ywRWUZ5bXyYHDL6maPBzJxfu5XLJTeEuDXZBc_CwIydjccSHacDnqLkLdvFYPeYE5udYQnNTH50fmSRCsvNxNDEVfCHqXAzxS1ZZzKlV9WLwUyJrs_nVfXt9uPXm8-L-y-f7m4-3C-wETIvUPTQK4mitQYV8U4YhKEzbWMl1EYAipUcrOzaAclYyfu6A4tFhbYF09Tiqro7cW0wG72LbmviQQfj9J9AiKM2MTucSLeD5NCB7JE3UlnqGjVgmUiNslbWvLDen1i7_arsgeSLYNMT6NOMd2s9hllzEAr6RhXC2zMhhh97SllvXUKaJuMp7JOuO8kliBaglDanUowhpUjDwxwO-ugJvdFnT-ijJ_TJE6XvzeNHPnT9dcC_LajIPjuKOqGj48-6SJiLLu4_I34DBebO5g</recordid><startdate>202306</startdate><enddate>202306</enddate><creator>Anwar, Laila</creator><creator>Ali, Syed Abid</creator><creator>Khan, Sana</creator><creator>Uzairullah, Mir Muhammad</creator><creator>Mustafa, Nazish</creator><creator>Ali, Urooj Anwer</creator><creator>Siddiqui, Faheema</creator><creator>Bhatti, Huma Aslam</creator><creator>Rehmani, Syed Jawad</creator><creator>Abbas, Ghulam</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-4774-2728</orcidid><orcidid>https://orcid.org/0000-0001-6559-766X</orcidid></search><sort><creationdate>202306</creationdate><title>Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates</title><author>Anwar, Laila ; Ali, Syed Abid ; Khan, Sana ; Uzairullah, Mir Muhammad ; Mustafa, Nazish ; Ali, Urooj Anwer ; Siddiqui, Faheema ; Bhatti, Huma Aslam ; Rehmani, Syed Jawad ; Abbas, Ghulam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-c390964c37dac6e183ac0f8a75d402a30c3b4fd487fcead419280dc844770a523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Anti-aging functional foods</topic><topic>Carbonyl trapping</topic><topic>Glycation</topic><topic>Trigonellafoenum-graecum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anwar, Laila</creatorcontrib><creatorcontrib>Ali, Syed Abid</creatorcontrib><creatorcontrib>Khan, Sana</creatorcontrib><creatorcontrib>Uzairullah, Mir Muhammad</creatorcontrib><creatorcontrib>Mustafa, Nazish</creatorcontrib><creatorcontrib>Ali, Urooj Anwer</creatorcontrib><creatorcontrib>Siddiqui, Faheema</creatorcontrib><creatorcontrib>Bhatti, Huma Aslam</creatorcontrib><creatorcontrib>Rehmani, Syed Jawad</creatorcontrib><creatorcontrib>Abbas, Ghulam</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Heliyon</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anwar, Laila</au><au>Ali, Syed Abid</au><au>Khan, Sana</au><au>Uzairullah, Mir Muhammad</au><au>Mustafa, Nazish</au><au>Ali, Urooj Anwer</au><au>Siddiqui, Faheema</au><au>Bhatti, Huma Aslam</au><au>Rehmani, Syed Jawad</au><au>Abbas, Ghulam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates</atitle><jtitle>Heliyon</jtitle><addtitle>Heliyon</addtitle><date>2023-06</date><risdate>2023</risdate><volume>9</volume><issue>6</issue><spage>e16866</spage><epage>e16866</epage><pages>e16866-e16866</pages><artnum>e16866</artnum><issn>2405-8440</issn><eissn>2405-8440</eissn><abstract>Senescence is a natural phenomenon of growing old. It accelerates under certain conditions like diabetes mellitus resulting in early decline of bodily functions, which can be avoided by many claimed functional foods. The present study aims to investigate the anti-aging ability of Fenugreek seeds (Trigonellafoenum-graecum); a common ingredient of Indo-Pak cuisines. Briefly, the Fenugreek seeds extract (FgSE) in concentrationsof0.1, 0.5 and 1 mg/ml inhibited the formation of Advanced Glycation End products (AGEs) and fructosamine adducts in Bovine serum albumin (BSA)/fructose model in vitro. The BSA conformational analysis via Circular Dichorism and Congo red assays showed that it preserves secondary structure of BSA in aforementioned model. Although mechanistic studies revealed insignificant lysine blocking ability of Fenugreek by OPA assay, however carbonyl entrapping was found to be 24%, 34% and 42% at 0.1, 0.5 and 1 mg/ml, respectively. In vivo model of High Fructose diet (HFD) induced glycation, FgSE treatment in doses of 10, 25 & 50 mg/kg markedly improved Escape latency (p < 0.01) and preserved cognition in Morris Water Maze. Our data further exhibits significant decrease of CML (Nε-carboxymethyl lysine) levels in serum and hippocampus byFgSE treatment in comparison with HFD group. Therefore, we deduced that FgSE prevents glycation-induced memory decline via entrapping the reactive carbonyl intermediates, formed during production of AGEs. Hence, as a promising functional food it slows down the harmful process of glycation and aging associated morbidities.
•Fenugreek seeds extract (FgSE) inhibited formation of advanced glycation end products.•Fenugreek seeds extract preserved the secondary structure of BSA in the presence of fructose.•Fenugreek seeds extract entrap the reactive carbonyl compounds required for the synthesis of AGEs.•Fenugreek seeds extract prevented the glycation-induced cognitive decline.•Fenugreek seeds extract reduced the CML levels in both hippocampus and serum of high fructose fed rats.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>37484294</pmid><doi>10.1016/j.heliyon.2023.e16866</doi><orcidid>https://orcid.org/0000-0002-4774-2728</orcidid><orcidid>https://orcid.org/0000-0001-6559-766X</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Anti-aging functional foods Carbonyl trapping Glycation Trigonellafoenum-graecum |
title | Fenugreek seed ethanolic extract inhibited formation of advanced glycation end products via scavenging reactive carbonyl intermediates |
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