In vitro regeneration of Didymopanax morototoni

The present study aimed at establishing a complete plant regeneration protocol for Didymopanax morototoni (matchwood), a native Brazilian forest species. Four types of explants (root, shoot, node, and cotyledonary leaves) were obtained from in vitro germinated seeds. In the first step, woody plant m...

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Bibliographic Details
Published in:Brazilian journal of biology 2006-05, Vol.66 (2A), p.455-462
Main Authors: Franco, E T H, Gavioli, L B, Ferreira, A G
Format: Article
Language:English
Subjects:
Araliaceae
Araliaceae - embryology
Araliaceae - physiology
BIOLOGY
Didymopanax
embryogenesis
plantlets
Regeneration - physiology
Schefflera (Didymopanax)
Seeds - embryology
Seeds - physiology
somatic embryo
woody plants
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Summary:The present study aimed at establishing a complete plant regeneration protocol for Didymopanax morototoni (matchwood), a native Brazilian forest species. Four types of explants (root, shoot, node, and cotyledonary leaves) were obtained from in vitro germinated seeds. In the first step, woody plant medium (WPM) with casein hydrolysate (250 mgL-1 ) and 2,4-D (1.0 and 5.0 mgL-1) were used combined with kinetin (0.1 and 1.0 mgL-1). Twenty days after inoculation, the material was evaluated. Embryogenic calli were split, transferred to expression medium with several combinations of NAA and KIN, and moved to fresh medium after 60 days. Light did not interfere in embryo expression. Somatic embryos were formed either from individual cells or cell clusters. Plantlets were obtained in WPM medium and 10 gL-1 of sucrose with no plant regulator, or using 0.1 mgL-1 BAP and 0.5 mgL-1GA. Plantlets from somatic embryos of D. morototoni developed in 33% of the cases.
ISSN:1519-6984
1678-4375
1519-6984
DOI:10.1590/S1519-69842006000300011