Tumor-associated hematopoietic stem and progenitor cells positively linked to glioblastoma progression

• Published in: Nature communications 2021-06, Vol.12 (1), p.3895-3895, Article 3895
• Main Authors: Lu, I-Na, Dobersalske, Celia, Rauschenbach, Laurèl, Teuber-Hanselmann, Sarah, Steinbach, Anita, Ullrich, Vivien, Prasad, Shruthi, Blau, Tobias, Kebir, Sied, Siveke, Jens T., Becker, Jürgen C., Sure, Ulrich, Glas, Martin, Scheffler, Björn, Cima, Igor
• Format: Article
• Language: English
• Subjects: 13; 13/100; 13/106; 13/21; 13/31; 13/51; 14/63; 38; 38/39; 38/91; 631/114/1314; 631/67/1922; 631/67/327; Brain; Brain cancer; Brain tumors; CD38 antigen; Cell culture; Cell cycle; Cell proliferation; Cytokines; Glioblastoma; Hematopoietic stem cells; Humanities and Social Sciences; Immune checkpoint; Immune system; Immunosuppression; Immunotherapy; Interleukin 6; Interleukin 8; Malignancy; Medical prognosis; Monocyte chemoattractant protein 1; multidisciplinary; Myelopoiesis; PD-L1 protein; Phenotypes; Progenitor cells; Science; Science (multidisciplinary); Stem cells; Target recognition; Tumor microenvironment; Tumors
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-23995-z

Brain tumors are typically immunosuppressive and refractory to immunotherapies for reasons that remain poorly understood. The unbiased profiling of immune cell types in the tumor microenvironment may reveal immunologic networks affecting therapy and course of disease. Here we identify and validate the presence of hematopoietic stem and progenitor cells (HSPCs) within glioblastoma tissues. Furthermore, we demonstrate a positive link of tumor-associated HSPCs with malignant and immunosuppressive phenotypes. Compared to the medullary hematopoietic compartment, tumor-associated HSPCs contain a higher fraction of immunophenotypically and transcriptomically immature, CD38- cells, such as hematopoietic stem cells and multipotent progenitors, express genes related to glioblastoma progression and display signatures of active cell cycle phases. When cultured ex vivo, tumor-associated HSPCs form myeloid colonies, suggesting potential in situ myelopoiesis. In experimental models, HSPCs promote tumor cell proliferation, expression of the immune checkpoint PD-L1 and secretion of tumor promoting cytokines such as IL-6, IL-8 and CCL2, indicating concomitant support of both malignancy and immunosuppression. In patients, the amount of tumor-associated HSPCs in tumor tissues is prognostic for patient survival and correlates with immunosuppressive phenotypes. These findings identify an important element in the complex landscape of glioblastoma that may serve as a target for brain tumor immunotherapies. A deeper knowledge of the immune cell profile within the brain cancer tumor microenvironment (TM) could identify targets to improve immunotherapy efficacy. Here, in glioblastoma, the authors find haematopoietic stem and progenitor cells in the TM, which are associated with poor prognosis and increased immunosuppression.