Galectin-1 Cooperates with Yersinia Outer Protein (Yop) P to Thwart Protective Immunity by Repressing Nitric Oxide Production

• Published in: Biomolecules (Basel, Switzerland) Switzerland), 2021-11, Vol.11 (11), p.1636
• Main Authors: Jofre, Brenda Lucila, Eliçabe, Ricardo Javier, Silva, Juan Eduardo, Pérez Sáez, Juan Manuel, Paez, Maria Daniela, Callegari, Eduardo, Mariño, Karina Valeria, Di Genaro, María Silvia, Rabinovich, Gabriel Adrián, Davicino, Roberto Carlos
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Apoptosis; Bacteria; Bacterial infections; Bacterial Outer Membrane Proteins - chemistry; Bacterial Outer Membrane Proteins - metabolism; Cell activation; Cell death; Cytokines; Cytoplasm; Galectin 1 - metabolism; Galectin-1; Immunity; Infections; Macrophages; MAP kinase; Mice; Mice, Inbred C57BL; Mice, Knockout; Nitric oxide; Nitric Oxide - biosynthesis; Peptide Hydrolases - metabolism; Protein Binding; Proteins; Proteolysis; Proteomics; Superoxide anions; T cell receptors; Trypsin; Tumor necrosis factor; Virulence; Virulence factors; Virulence Factors - metabolism; Yersinia enterocolitica; Yersinia enterocolitica - immunology; Yersinia enterocolitica - pathogenicity; YopP
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom11111636

(Ye) inserts outer proteins (Yops) into cytoplasm to infect host cells. However, in spite of considerable progress, the mechanisms implicated in this process, including the association of Yops with host proteins, remain unclear. Here, we evaluated the functional role of Galectin-1 (Gal1), an endogenous β-galactoside-binding protein, in modulating Yop interactions with host cells. Our results showed that Gal1 binds to Yops in a carbohydrate-dependent manner. Interestingly, Gal1 binding to Yops protects these virulence factors from trypsin digestion. Given that early control of Ye infection involves activation of macrophages, we evaluated the role of Gal1 and YopP in the modulation of macrophage function. Although Gal1 and YopP did not influence production of superoxide anion and/or TNF by Ye-infected macrophages, they coordinately inhibited nitric oxide (NO) production. Notably, recombinant Gal1 (rGal1) did not rescue NO increase observed in macrophages infected with the YopP mutant Ye ∆ . Whereas NO induced apoptosis in macrophages, no significant differences in cell death were detected between Gal1-deficient macrophages infected with Ye ∆ , and WT macrophages infected with Ye wt. Strikingly, increased NO production was found in WT macrophages treated with MAPK inhibitors and infected with Ye wt. Finally, rGal1 administration did not reverse the protective effect in Peyer Patches (PPs) of mice infected with Ye ∆ . Our study reveals a cooperative role of YopP and endogenous Gal1 during Ye infection.