Synergistic Effects of Lauryl Gallate and Tamoxifen on Human Breast Cancer Cell

Tamoxifen (TAM) is widely used for adjuvant therapy in breast cancer patients. Tamoxifen therapy may lead to serious side effects. Anti-apoptotic substances in combination with chemotherapy drugs can result in additive or synergistic effects. Lauryl gallate (LG), a Gallic acid derivative, has been p...

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Bibliographic Details
Published in:Iranian journal of public health 2020-07, Vol.49 (7), p.1324-1329
Main Authors: Ghatreh Samani, Keihan, Farrokhi, Effat, Tabatabaee, Aliye, Jalilian, Narges, Jafari, Mahbube
Format: Article
Language:English
Subjects:
Apoptosis
Bcl-2 protein
Breast Cancer
Chemotherapy
Flow cytometry
Gallic acid
Gene expression
Lauryl gallate
Medical science
Original
Side effects
SIRT1 protein
Synergistic effect
Tamoxifen
Tumors
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Summary:Tamoxifen (TAM) is widely used for adjuvant therapy in breast cancer patients. Tamoxifen therapy may lead to serious side effects. Anti-apoptotic substances in combination with chemotherapy drugs can result in additive or synergistic effects. Lauryl gallate (LG), a Gallic acid derivative, has been proven to inhibit tumor growth, without affecting normal cells. This study aimed to investigate the synergistic effect of TAM and LG in breast cancer cell line (MCF-7). In this experimental study, performed in ShahreKord University of Medical Science, Iran in 2017, the MCF-7 cells were treated by final concentrations of 10 μM TAM alone, and in combination with 200 μM of LG. We also used EX-527, as SIRT-1 inhibitor to examine the role of SIRT1 in cell apoptosis. and gene expression were measured by real-time PCR method, and cell apoptosis was investigated by flow cytometry. Tamoxifen alone and in combination with LG decreased expression 2.64±0.75 and 6.38±1.9 fold, respectively, after 48 h (
ISSN:2251-6085
2251-6093
DOI:10.18502/ijph.v49i7.3586