Chitinase Is Involved in the Fruiting Body Development of Medicinal Fungus Cordyceps militaris

is a famous traditional edible and medicinal fungus in Asia, and its fruiting body has rich medicinal value. The molecular mechanism of fruiting body development is still not well understood in . In this study, phylogenetically analysis and protein domains prediction of the 14 putative chitinases we...

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Bibliographic Details
Published in:Life (Basel, Switzerland) Switzerland), 2023-03, Vol.13 (3), p.764
Main Authors: Zhang, Zi-Juan, Yin, Yuan-Yuan, Cui, Yao, Zhang, Yue-Xuan, Liu, Bi-Yang, Ma, You-Chu, Liu, Yong-Nan, Liu, Gao-Qiang
Format: Article
Language:English
Subjects:
Bioinformatics
cell wall
Cell walls
Chitin
chitin degrading enzyme
Chitinase
Cordyceps militaris
Enzymatic activity
Enzyme activity
Enzymes
Fruit bodies
Fungi
Gene silencing
Genes
Glucose
mushroom formation
Mushrooms
Phylogeny
Proteins
RNA-mediated interference
Signal transduction
Trace elements
Variance analysis
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Summary:is a famous traditional edible and medicinal fungus in Asia, and its fruiting body has rich medicinal value. The molecular mechanism of fruiting body development is still not well understood in . In this study, phylogenetically analysis and protein domains prediction of the 14 putative chitinases were performed. The transcription level and enzyme activity of chitinase were significant increased during fruiting body development of Then, two chitinase genes ( and ) were selected to construct gene silencing strain by RNA interference. When and genes were knockdown, the differentiation of the primordium was blocked, and the number of fruiting body was significantly decreased approximately by 50% compared to wild-type (WT) strain. The length of the single mature fruiting body was shortened by 27% and 38% in and silenced strains, respectively. In addition, the chitin content and cell wall thickness were significantly increased in and silenced strains. These results provide new insights into the biological functions of chitinase in fruiting body development of
ISSN:2075-1729
2075-1729
DOI:10.3390/life13030764