Silver Nanocomposite Biosynthesis: Antibacterial Activity against Multidrug-Resistant Strains of Pseudomonas aeruginosa and Acinetobacter baumannii

• Published in: Molecules (Basel, Switzerland) Switzerland), 2016-09, Vol.21 (9), p.1255
• Main Authors: Silva Santos, Klebson, Barbosa, Andriele Mendonça, Pereira da Costa, Luiz, Pinheiro, Malone Santos, Oliveira, Maria Beatriz Prior Pinto, Ferreira Padilha, Francine
• Format: Article
• Language: English
• Subjects: Acinetobacter baumannii; antibacterial activity; multidrug-resistance; Pseudomonas aeruginosa; silver nanocomposite biosynthesis
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules21091255

Bacterial resistance is an emerging public health issue that is disseminated worldwide. Silver nanocomposite can be an alternative strategy to avoid Gram-positive and Gram-negative bacteria growth, including multidrug-resistant strains. In the present study a silver nanocomposite was synthesized, using a new green chemistry process, by the addition of silver nitrate (1.10 mol·L ) into a fermentative medium of spp. to produce a xanthan gum polymer. Transmission electron microscopy (TEM) was used to evaluate the shape and size of the silver nanoparticles obtained. The silver ions in the nanocomposite were quantified by flame atomic absorption spectrometry (FAAS). The antibacterial activity of the nanomaterial against (ATCC 22652), (ATCC 29282), (ATCC 27853) and (ATCC 25923) was carried out using 500 mg of silver nanocomposite. and multidrug-resistant strains, isolated from hospitalized patients were also included in the study. The biosynthesized silver nanocomposite showed spherical nanoparticles with sizes smaller than 10 nm; 1 g of nanocomposite contained 49.24 µg of silver. Multidrug-resistant strains of and , and the other Gram-positive and Gram-negative bacteria tested, were sensitive to the silver nanocomposite (10-12.9 mm of inhibition zone). The biosynthesized silver nanocomposite seems to be a promising antibacterial agent for different applications, namely biomedical devices or topical wound coatings.