Micro-Volume Blood Separation Membrane for In-Situ Biosensing

In this paper, we report a point-of-care (POCT) testing strip based on a porous membrane structure for whole blood separation and colorimetric analysis without external supporting equipment. Conventional blood tests rely on large instruments for blood pretreatment and separation to improve measureme...

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Bibliographic Details
Published in:Biosensors (Basel) 2022-09, Vol.12 (9), p.712
Main Authors: Zhu, Qin, Wu, Huimin, Ma, Zhen, Liu, Yuqiao, Li, Junmin, Zhu, Ling, Zhang, Xinran, Wang, Chengcheng, Chen, Dajing, Zhu, Danhua
Format: Article
Language:English
Subjects:
Acetic acid
Analysis
Biomarkers
Biosensors
Blood cells
blood separation
Cellulose acetate
colorimetric assay
Colorimetry
Communication
Diameters
Fluid dynamics
Fluid flow
Fourier transforms
Glucose
Health aspects
Laboratories
Membrane structure
Membrane structures
Membranes
Microfluidics
Morphology
Plasma
Polyethylene glycol
Pores
Porous media flow
porous membranes
Reagents
Response time
Screen printing
Separation
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Summary:In this paper, we report a point-of-care (POCT) testing strip based on a porous membrane structure for whole blood separation and colorimetric analysis without external supporting equipment. Conventional blood tests rely on large instruments for blood pretreatment and separation to improve measurement accuracy. Cellulose acetate (CA) membranes with different pore diameters and structures were prepared via a non-solvent method for the separation of whole blood. Among them, CA@PEG-2000 membranes with nano-pores on the surface and micro-pores in the interior facilitated the capture of blood cells on the surface, as well as the free diffusion of plasma through the porous interior structure. The fluid flow of blood in the asymmetric porous structure can be theoretically estimated using the Lucas-Washburn equation. Compared with the conventional paper strips and other porous membranes, the CA@PEG-2000 membrane with an immobilized sensing layer exhibited efficient blood separation, a short response time (less than 2 min), an ultralow dosage volume (5 μL), and high sensitivity. The fabricated blood separation membranes can be further used for the detection of various biomarkers in whole blood, providing additional options for rapid quantitative POCT tests.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12090712