Low LINC02147 expression promotes the malignant progression of oral submucous fibrosis

Background Key lncRNAs associated with the malignant progression of oral submucous fibrosis (OSF) to oral squamous cell carcinoma (OSCC) were identified. Methods Key lncRNAs with sequential changes from normal oral mucosa (NOM) to OSF to OSCC were identified based on the GEO database. Kaplan-Meier a...

Full description

Saved in:
Bibliographic Details
Published in:BMC oral health 2022-07, Vol.22 (1), p.1-316, Article 316
Main Authors: Chen, Jun, Li, Wenjie, Liu, Binjie, Xie, Xiaoli
Format: Article
Language:English
Subjects:
Analysis
Bioinformatics
Biological markers
Biomarker
Care and treatment
Cell differentiation
Cell proliferation
Cell viability
Diagnosis
Ethics
Fibrosis
Fluorescence in situ hybridization
Gene expression
Gene set enrichment analysis
Genetic susceptibility
Genomes
lncRNA
Medical prognosis
Mouth cancer
Nomograms
Non-coding RNA
Oral cancer
Oral carcinoma
Oral squamous cell carcinoma
Oral squamous cell carcinoma (OSCC)
Oral submucous fibrosis (OSF)
Pathogenesis
Prognosis
Risk factors
Squamous cell carcinoma
Survival analysis
Testing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Key lncRNAs associated with the malignant progression of oral submucous fibrosis (OSF) to oral squamous cell carcinoma (OSCC) were identified. Methods Key lncRNAs with sequential changes from normal oral mucosa (NOM) to OSF to OSCC were identified based on the GEO database. Kaplan-Meier analysis was used to screen lncRNAs related to OSCC prognosis. Cox regression analysis was used to validate the independent prognostic value. qPCR was used to confirm the expression of the candidate lncRNAs. Gene set enrichment analysis (GSEA), nucleocytoplasmic separation assay, fluorescence in situ hybridization, RNA knockdown, western blot, and cell viability assay were performed to investigate the biological functions of the candidate lncRNA. A nomogram was constructed to quantitatively predict OSCC prognosis based on TCGA. Results Bioinformatics methods indicated that LINC02147 was sequentially downregulated from NOM to OSF to OSCC, as confirmed by clinical tissues and cells. Meanwhile, low LINC02147 expression, as an independent prognostic factor, predicted a poor prognosis for OSCC. GSEA and in vitro studies suggested that low LINC02147 expression promoted OSF malignant progression by promoting cell proliferation and differentiation. A LINC02147 signature-based nomogram successfully quantified each indicator's contribution to the overall survival of OSCC. Conclusions Low LINC02147 expression promoted OSF malignant progression and predicted poor OSCC prognosis. Keywords: Oral submucous fibrosis (OSF), Oral squamous cell carcinoma (OSCC), lncRNA, Biomarker, Prognosis
ISSN:1472-6831
1472-6831
DOI:10.1186/s12903-022-02346-4