Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles

A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by t...

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Bibliographic Details
Published in:Toxins 2015-12, Vol.7 (12), p.5377-5385
Main Authors: Luan, Yunxia, Chen, Jiayi, Li, Cheng, Xie, Gang, Fu, Hailong, Ma, Zhihong, Lu, Anxiang
Format: Article
Language:English
Subjects:
Alcoholic Beverages - analysis
aptamer
Aptamers, Nucleotide - chemistry
cationic polymer
colorimetric assay
Colorimetry
Food Contamination - analysis
Gold - chemistry
gold nanoparticles
Metal Nanoparticles - chemistry
Ochratoxin A (OTA)
Ochratoxins - analysis
Ochratoxins - chemistry
Polyethylenes - chemistry
Quaternary Ammonium Compounds - chemistry
Sodium Chloride - chemistry
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Summary:A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins7124883