Staying below the Radar: Unraveling a New Family of Ubiquitous "Cryptic" Non-Tailed Temperate Vibriophages and Implications for Their Bacterial Hosts

• Published in: International journal of molecular sciences 2023-02, Vol.24 (4), p.3937
• Main Authors: Kalatzis, Panos G, Mauritzen, Jesper Juel, Winther-Have, Caroline Sophie, Michniewski, Slawomir, Millard, Andrew, Tsertou, Maria Ioanna, Katharios, Pantelis, Middelboe, Mathias
• Format: Article
• Language: English
• Subjects: Bacteria; Bacteriophages - genetics; Biofilms; Cell density; dif sites; Genes; Genome, Bacterial; Genomes; Genomic analysis; Genomics; Geographical distribution; Infections; integration; Lysogeny; Microscopy; Morphology; NO16; non-tailed phages; Oceans; Phages; Phylogenetics; Predation; Prophages; Prophages - genetics; Proteins; Recombination; Taxonomy; ubiquitous presence; Vibrio; Vibrio - genetics; Virulence; Viruses
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24043937

Bacteriophages are the most abundant biological entities in the oceans and play key roles in bacterial activity, diversity and evolution. While extensive research has been conducted on the role of tailed viruses (Class: ), very little is known about the distribution and functions of the non-tailed viruses (Class: ). The recent discovery of the lytic family demonstrated the potential importance of this structural lineage, emphasizing the need for further exploration of the role of this group of marine viruses. Here, we report the novel family of temperate phages under the class of , which we propose to name "Asemoviridae" with phage NO16 as a main representative. These phages are widely distributed across geographical regions and isolation sources and found inside the genomes of at least 30 species of , in addition to the original isolation host. Genomic analysis identified -like sites, suggesting that NO16 prophages recombine with the bacterial genome based on the XerCD site-specific recombination mechanism. The interactions between the NO16 phage and its host were linked to cell density and phage-host ratio. High cell density and low phage predation levels were shown to favor the temperate over the lytic lifestyle for NO16 viruses, and their spontaneous induction rate was highly variable between different lysogenic strains. NO16 prophages coexist with the host in a mutualistic interaction by rendering fitness properties to the host, such as increased virulence and biofilm formation through lysogenic conversion, likely contributing to their global distribution.