Functional diversity of genes for the biosynthesis of paeoniflorin and its derivatives in Paeonia

The Paeonia root, with or without bark, are considered vital traditional Chinese medicine materials; the examples are those of Bai Shao, Chi Shao, and Dan Pi. In this study, we examine 24 genes and their expressions involved in the biosynthesis of paeoniflorin and its derivatives, which are active c...

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Published in:International journal of molecular sciences 2013-09, Vol.14 (9), p.18502-18519
Main Authors: Yuan, Yuan, Yu, Jun, Jiang, Chao, Li, Minhui, Lin, Shufang, Wang, Xumin, Huang, Luqi
Format: Article
Language:English
Subjects:
and gallic acid
bark
Benzoates - metabolism
Biosynthesis
Bridged-Ring Compounds - metabolism
Chinese medicine
Coriaria
Dehydrogenases
Enzymes
Gene expression
Glucosides - metabolism
Kinases
Monoterpenes
orthologs
Paeonia
Paeonia - metabolism
paeoniflorin
Phylogenetics
Phylogeny
Plant Proteins - classification
Plant Proteins - metabolism
Punica granatum
Rheumatoid arthritis
Rhus radicans
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Summary:The Paeonia root, with or without bark, are considered vital traditional Chinese medicine materials; the examples are those of Bai Shao, Chi Shao, and Dan Pi. In this study, we examine 24 genes and their expressions involved in the biosynthesis of paeoniflorin and its derivatives, which are active compounds of the Paeonia root, in Paeonia lactiflora and P. suffruticosa, as well as other related plants, Punica granatum, Rhus radicans, and Coriaria nepalensis. Our phylogenetic analyses suggest that these genes have functional diversity, and analysis of the transcriptional level shows paeoniflorin and gallic acid biosynthesis-related genes exhibit different transcription profiles in flowers, carpels, bark-free roots, and bark of P. lactiflora. The correlation analysis of gene expression and active compound contents support the idea that hydroxymethylglutaryl-CoA synthase and phosphomevalonate kinase in the mevalonate pathway and 3-dehydroquinate dehydratase/shikimate dehydrogenase in shikimate biosynthesis are potentially closely related to the accumulation of paeoniflorin and benzoylpaeoniflorin. Coupling gene diversity with chemical analysis, we show that paeoniflorin and its derived aromatic amino acids are predominant in bark.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms140918502