Spinning around or stagnation - what do osteoblasts and chondroblasts really like?

The influence of cytomechanical forces in cellular migration, proliferation and differentiation of mesenchymal stem cells (MSCs) is still poorly understood in detail. Human MSCs were isolated and cultivated onto the surface of a 3 x 3 mm porcine collagen I / III carrier. After incubation, cell cultu...

Full description

Saved in:
Bibliographic Details
Published in:European journal of medical research 2010-01, Vol.15 (1), p.35-43
Main Authors: Zilkens, C, Lögters, T, Bittersohl, B, Krauspe, R, Lensing-Höhn, S, Jäger, Marcus
Format: Article
Language:English
Subjects:
Biomarkers
Bone-grafting
Cell Culture Techniques - methods
Cell Differentiation - physiology
Cell proliferation
Cells, Cultured
chondroblast
Chondrocytes - cytology
Chondrocytes - physiology
Chondrogenesis - physiology
Culture Media - pharmacology
cytomechanical forces
differentiation
Gene expression
Gene Expression Profiling
Humans
Immunohistochemistry
mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Methods
osteoblast
Osteoblasts
Osteoblasts - cytology
Osteoblasts - physiology
Osteogenesis - physiology
Physiological aspects
Reverse Transcriptase Polymerase Chain Reaction
Stress, Mechanical
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The influence of cytomechanical forces in cellular migration, proliferation and differentiation of mesenchymal stem cells (MSCs) is still poorly understood in detail. Human MSCs were isolated and cultivated onto the surface of a 3 x 3 mm porcine collagen I / III carrier. After incubation, cell cultures were transferred to the different cultures systems: regular static tissue flasks (group I), spinner flasks (group II) and rotating wall vessels (group III). Following standard protocols cells were stimulated lineage specific towards the osteogenic and chondrogenic lines. To evaluate the effects of applied cytomechanical forces towards cellular differentiation distinct parameters were measured (morphology, antigen and antigen expression) after a total cultivation period of 21 days in vitro. Depending on the cultivation technique we found significant differences in both gen and protein expression. Cytomechanical forces with rotational components strongly influence the osteogenic and chondrogenic differentiation.
ISSN:0949-2321
2047-783X
2047-783X
DOI:10.1186/2047-783X-15-1-35