Dual-Antigen Subunit Vaccine Nanoparticles for Scrub Typhus

is the causative pathogen of scrub typhus, an acute febrile disease prevalent in the Asia-Pacific region that is spread to people through chigger bites. Despite the emerging threat, there is no currently available vaccine against . Here, we developed dual-antigen subunit vaccine nanoparticles using...

Full description

Saved in:
Bibliographic Details
Published in:Pathogens (Basel) 2023-11, Vol.12 (12), p.1390
Main Authors: Park, Jaeyoung, Zhang, Zhiwen, Belinskaya, Tatyana, Tsoras, Alexandra N, Chao, Chien-Chung, Jiang, Le, Champion, Julie A
Format: Article
Language:English
Subjects:
acetone
Amino acids
Antibodies
Antigens
Bacterial vaccines
Biocompatibility
chiggers
Dosage and administration
Health aspects
Immune response
Immune response (cell-mediated)
immunity
Immunogenicity
Influenza
nanoparticle
Nanoparticles
Orientia
Orientia tsutsugamushi
pathogens
Polyclonal antibodies
Polyethylene glycol
Polymers
Prevention
Proteins
Scrub typhus
subunit vaccines
Testing
Typhus
vaccine
Vaccines
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:is the causative pathogen of scrub typhus, an acute febrile disease prevalent in the Asia-Pacific region that is spread to people through chigger bites. Despite the emerging threat, there is no currently available vaccine against . Here, we developed dual-antigen subunit vaccine nanoparticles using recombinant 47 kD and 56 kD proteins, which are immunogenic outer membrane antigens of The biocompatible protein vaccine nanoparticles were formed via desolvation of r56 or r47E antigens with acetone, coating with an additional layer of the 56 kD protein, and stabilization with reducible homobifunctional DTSSP and heterobifunctional SDAD crosslinkers. The dual-antigen subunit vaccine nanoparticles significantly improved antigen-specific antibody responses in vaccinated mice. Most importantly, the dual-antigen nanoparticles coated with an additional layer of the 56 kD protein were markedly more immunogenic than soluble antigens or single-antigen nanoparticles in the context of cellular immune responses. Given the significance of cellular immune responses for protection against , these results demonstrate the potent immunogenicity of dual-layered antigen nanoparticles and their potential as a promising strategy for developing vaccines against scrub typhus.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens12121390