Expression of CIB1 correlates with colorectal liver metastases but not with peritoneal carcinomatosis

Molecular differences in colorectal cancer (CRC) are associated with the metastatic route. Patient survival is mainly driven by metastatic spread thus it is imperative to understand its key drivers to develop biomarkers for risk stratification, follow-up protocols and personalized therapy. Thus, thi...

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Bibliographic Details
Published in:BMC cancer 2021-11, Vol.21 (1), p.1243-1243, Article 1243
Main Authors: Jacob, Sven, Bösch, Florian, Schoenberg, Markus B, Pretzsch, Elise, Lampert, Christopher, Haoyu, Ren, Renz, Bernhard W, Michl, Marlies, Kumbrink, Jörg, Kirchner, Thomas, Werner, Jens, Angele, Martin K, Neumann, Jens
Format: Article
Language:English
Subjects:
Aged
Angiogenesis
Biomarkers
Calcium-binding proteins
Calcium-Binding Proteins - genetics
Cancer
Colonic Neoplasms - genetics
Colonic Neoplasms - pathology
Colorectal cancer
Colorectal carcinoma
Development and progression
Epithelial-Mesenchymal Transition - genetics
Female
Gastric cancer
Gene expression
Gene Expression Profiling
Genetic aspects
Health aspects
Humans
Immunohistochemistry
Laboratories
Liver
Liver cancer
Liver Neoplasms - secondary
Male
Medical research
Mesenchyme
Metastases
Metastasis
Neoplasm Proteins - genetics
Neovascularization, Pathologic - genetics
Paraffin
Patients
Peritoneal Neoplasms - secondary
Peritoneum
Principal components analysis
Risk factors
Software
Tumors
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Summary:Molecular differences in colorectal cancer (CRC) are associated with the metastatic route. Patient survival is mainly driven by metastatic spread thus it is imperative to understand its key drivers to develop biomarkers for risk stratification, follow-up protocols and personalized therapy. Thus, this study aimed to identify genes associated with the metastatic route in CRC. CRC patients resected at our clinic from 2005 to 2014 and with a minimum 5-year follow-up were included in this analysis and grouped into CRC with hepatic (HEP), peritoneal (PER) or without distant metastases (M0), and HEP/PER. Firstly, tumor RNA of 6 patients each was isolated by microdissection from formalin-fixed paraffin-embedded specimens and analyzed by a NanoString analysis. Subsequently, these results were validated with immunohistochemistry and correlated to clinicopathological parameters in a larger collective of CRC patients (HEP n = 51, PER n = 44, M0 n = 47, HEP/PER n = 28). Compared to M0, HEP tumors showed 20 differentially expressed genes associated with epithelial-mesenchymal transition (EMT) and angiogenesis. Compared to M0, PER tumors had 18 differentially expressed genes. The finding of different gene signatures was supported by the multidimensional principal component clustering analysis. Tumor perforation did not influence the metastatic route. CIB1 was homogenously and significantly overexpressed in HEP compared to M0 (p 
ISSN:1471-2407
1471-2407
DOI:10.1186/s12885-021-08927-w