Detection and quantification of human adenovirus genomes in Acanthamoeba isolated from swimming pools

Acanthamoeba is the most common free-living environmental amoeba, it may serve as an important vehicle for various microorganisms living in the same environment, such as viruses, being pathogenic to humans. This study aimed to detect and quantify human adenoviruses (HAdV) in Acanthamoebas isolated f...

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Published in:Anais da Academia Brasileira de Ciências 2016-01, Vol.88 Suppl 1 (suppl 1), p.635-641
Main Authors: Staggemeier, Rodrigo, Arantes, Thalita, Caumo, Karin S, Rott, Marilise B, Spilki, Fernando R
Format: Article
Language:English
Subjects:
Acanthamoeba
Acanthamoeba - virology
Adenoviruses, Human - genetics
adenovírus humano
ameba de vida livre
Brazil
Genome, Viral
Humans
MULTIDISCIPLINARY SCIENCES
piscinas
qPCR
Swimming Pools
Water Microbiology
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Summary:Acanthamoeba is the most common free-living environmental amoeba, it may serve as an important vehicle for various microorganisms living in the same environment, such as viruses, being pathogenic to humans. This study aimed to detect and quantify human adenoviruses (HAdV) in Acanthamoebas isolated from water samples collected from swimming pools in the city of Porto Alegre, Southern Brazil. Free-living amoebae of the genus Acanthamoeba were isolated from water samples, and isolates (n=16) were used to investigate the occurrence of HAdVs. HAdV detection was performed by quantitative real-time polymerase chain reaction (qPCR). HAdVs were detected in 62.5% (10/16) of Acanthamoeba isolates, ranging from 3.24x103 to 5.14x105 DNA copies per milliliter of isolate. HAdV viral loads found in this study are not negligible, especially because HAdV infections are associated with several human diseases, including gastroenteritis, respiratory distress, and ocular diseases. These findings reinforce the concept that Acanthamoeba may act as a reservoir and promote HAdV transmission through water.
ISSN:0001-3765
1678-2690
1678-2690
DOI:10.1590/0001-3765201620150151