Detection of Spiked Fasciola hepatica Eggs in Stool Specimens Using LAMP Technique

Fascioliasis is one of the most important food-borne worm disease caused by sp. Parasitological diagnosis is more difficult due to the low parasite burden and a few eggs shedding of helminths. Therefore, it will be valuable to development of simple, fast and reliable diagnostic tests for detection o...

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Bibliographic Details
Published in:Iranian journal of parasitology 2019-07, Vol.14 (3), p.387-393
Main Authors: Ghodsian, Sahar, Rouhani, Soheila, Fallahi, Shirzad, Seyyedtabaei, Seyyed Javad, Taghipour, Niloofar
Format: Article
Language:English
Subjects:
Eggs
Fasciola hepatica
LAMP
Original
Spiked egg
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Summary:Fascioliasis is one of the most important food-borne worm disease caused by sp. Parasitological diagnosis is more difficult due to the low parasite burden and a few eggs shedding of helminths. Therefore, it will be valuable to development of simple, fast and reliable diagnostic tests for detection of human and animal fascioliasis. Infected liver collected from abattoir in Tehran, Iran in 2017. eggs were detached from the uterus of worms under a stereo microscope. Various numbers of eggs were spiked to 200 mgr. of negative feces samples. DNA was extracted and then target regions (nuclear IGS) were amplified by LAMP assay using six primers. Fecal specimens without egg and DNA of other helminths were used as negative controls. sample which confirmed by morphologic criteria and PCR-RFLP was used as positive control. LAMP products by using SYBR Green I could detect even a single egg in fecal samples which was visible by change of color from orange to green. There was no cross amplification by other helminths including; and . LAMP seems a rapid, sensitive, cost-effective technique for detection of human fascioliasis.
ISSN:1735-7020
2008-238X
DOI:10.18502/ijpa.v14i3.1477