Immunohistochemical Studies of Age-Related Changes in Cell Proliferation and Angiogenesis during the Healing of Acetic Acid-Induced Gastric Ulcers in Rats

Cell proliferation and angiogenesis are of utmost importance for healing to take place. The KI67 and EGFR proteins are markers of cell proliferation, while CD31 and factor VIII are markers of angiogenesis. To elucidate the mechanism responsible for delayed healing of the gastric injury in old age, w...

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Bibliographic Details
Published in:TheScientificWorld 2020, Vol.2020 (2020), p.1-10
Main Authors: Ajayi, A. Folorunsho, Olaleye, S. Babafemi
Format: Article
Language:English
Subjects:
Acetic Acid
Acids
Age
Age Factors
Aging
Angiogenesis
Animal tissues
Animals
Biomarkers - metabolism
Blood coagulation factor VIII
Cell growth
Cell proliferation
Cell Proliferation - physiology
Coagulation factors
Epidermal growth factor receptors
Epithelial cells
ErbB Receptors - biosynthesis
Factor VIII - biosynthesis
Gastric mucosa
Growth factors
Healing
Immunohistochemistry - methods
Injury analysis
Ki-67 Antigen - biosynthesis
Kinases
Male
Markers
Neovascularization, Physiologic - physiology
Organic acids
Platelet Endothelial Cell Adhesion Molecule-1 - biosynthesis
Potassium
Proteins
Rats, Wistar
Stomach
Stomach Ulcer - chemically induced
Stomach Ulcer - metabolism
Stomach Ulcer - physiopathology
Ulcers
Wound Healing - physiology
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Summary:Cell proliferation and angiogenesis are of utmost importance for healing to take place. The KI67 and EGFR proteins are markers of cell proliferation, while CD31 and factor VIII are markers of angiogenesis. To elucidate the mechanism responsible for delayed healing of the gastric injury in old age, we analyzed the expression of these markers in rats of different months during the healing of an acetic acid-induced gastric ulcer. Male Wistar rats (aged 3, 6, 12, and 18 months) divided into four groups, according to their ages, formed the experimental animals. Stomach tissue samples were collected on days 3, 7, 14, and 21 after induction for assessment of ulcer healing. The area of gastric mucosa healed was inversely proportional to age. The expression of markers of proliferation (KI67 and EGFR) and angiogenesis (factor VIII and CD31) decreased significantly (p six months > 12 months > 18 months) on days 7, 14, and 21 after induction of gastric ulcer. This study revealed that the slower gastric ulcer healing rate in older rats might be due to reduced epithelial cell proliferation and angiogenic activities.
ISSN:2356-6140
1537-744X
1537-744X
DOI:10.1155/2020/3506207