Transferred Cold Atmospheric Plasma Treatment on Melanoma Skin Cancer Cells with/without Catalase Enzyme In Vitro

Cold atmospheric plasma (CAP) is a promising tool to overcome certain cancerous and precancerous conditions in dermatology. A scheme of transferred CAP was first developed to treat melanoma (B16F10) skin cancer cells as well as non-malignant (L929) cells in vitro. CAP was transferred using a silicon...

Full description

Saved in:
Bibliographic Details
Published in:Applied sciences 2021-07, Vol.11 (13), p.6181
Main Authors: Chen, Yun-Hsuan, Hsieh, Jang-Hsing, Wang, I-Te, Jheng, Pei-Ru, Yeh, Yi-Yen, Lee, Jyh-Wei, Bolouki, Nima, Chuang, Er-Yuan
Format: Article
Language:English
Subjects:
Apoptosis
Atmospheric pressure
Cancer therapies
Catalase
cold atmospheric plasma (CAP)
Cytotoxicity
Dermatology
Gases
Hair loss
Medical research
Melanoma
melanoma cell (B16F10)
Nitrogen
Plasma
plasma cancer therapy
Radiation therapy
reactive nitrogen species (RNS)
reactive oxygen species (ROS)
Room temperature
Silicones
Skin cancer
transferred cold atmospheric plasma
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cold atmospheric plasma (CAP) is a promising tool to overcome certain cancerous and precancerous conditions in dermatology. A scheme of transferred CAP was first developed to treat melanoma (B16F10) skin cancer cells as well as non-malignant (L929) cells in vitro. CAP was transferred using a silicone tube with a jet system that was developed and was assessed as to whether it could generate reactive oxygen and nitrogen species (RONS) at near-room temperature. The transferred CAP was characterized electrically and spectroscopically. Biological data showed that the transferred CAP killed cancer cells but not non-malignant (L929) cells. Plasma treatment was effective with a time duration of 30 s, whereas non-malignant (L929) cells were less damaged during plasma treatment. In addition, catalase (CAT) enzyme was applied to neutralize and detoxify the RONS generated by the transferred CAP. These findings suggest that transferred CAP can be considered a melanoma cancer therapy.
ISSN:2076-3417
2076-3417
DOI:10.3390/app11136181