Structural Characterization and Immunobiological Activity of Polysaccharides from Astragalus Oyster Mushroom

• Published in: Molecules (Basel, Switzerland) Switzerland), 2023-07, Vol.28 (13), p.5280
• Main Authors: Meng, Qiuxia, Niu, Yu, Wang, Rongrong, Niu, Wei, Zhang, Lizhen
• Format: Article
• Language: English
• Subjects: Arabinose; Astragalus Plant - chemistry; Biological activity; Cancer; Cellulose; Chromatography; Column chromatography; Cytotoxicity; D-Galactose; Dextran; Dextrans; Dialysis; Drug dosages; Fatty acids; Functional foods; Functional foods & nutraceuticals; Galactose; Glucose; Hengshan Astragalus oyster mushroom; Herbal medicine; Hydrogen Peroxide; Immunomodulation; Macrophages; Mannose; MAP kinase; Medical research; Medicinal herbs; Molecular weight; Monosaccharides; Mushrooms; NF-κB protein; Oysters; Pleurotus - chemistry; polysaccharide; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - pharmacology; Renal Dialysis; Retention; Saccharides; Structural analysis; Substrates; Tumor Necrosis Factor-alpha; Tumor necrosis factor-α
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28135280

When added to mushroom growing substrates, edible and medicinal herbs affect the mushrooms' nutritional and medicinal value. In this study, polysaccharides (P OP-I and P OP-I) were extracted and purified from oyster mushrooms grown on substrates supplemented with 0% and 15% Astragalus roots (P and P ), respectively, and their chemical structure and immunobiological activities were compared. P OP-I and P OP-I were extracted using ultrasound-assisted hot water and deproteinized with the Sevage method, depigmented with 30% H O , desalted with dialysis, and purified using DEAE-52 cellulose and Sephadex G-100 dextran column chromatography. The molecular weight of P OP-I and P OP-I was 21,706.96 and 20,172.65 Da, respectively. Both were composed of monosaccharides -mannose, galacturonic acid, -glucose, -galactose, and -arabinose but in different molar ratios, and both were connected by a pyranoside linkage. P OP-I consisted of higher contents of mannose, glucose, galactose and arabinose and lower content of galacturonic acid as compared to P OP-I. Both P OP-I and P OP-I induced NO and TNF-α production but did not show cytotoxic effect or induce ROS generation in RAW264.7 cells. P OP-I showed a stronger ability to promote NO and TNF-α production relative to P OP-I. In vitro experiments showed that the immunomodulatory activity of P OP-I and P OP-I in RAW264.7 macrophages were mediated by the JNK/MAPK, Erk/MAPK, and NF-κB signaling pathways. The results would be helpful for elucidation of the health promoting mechanism of Astragalus oyster mushrooms as a source of neutraceuticals.