TETRASPANIN 8-1 from Phaseolus vulgaris plays a key role during mutualistic interactions

Arbuscular mycorrhizal (AM) fungi and rhizobia form two of the most important plant-microbe associations for the assimilation of phosphorus (P) and nitrogen (N). Symbiont-derived signals are able to coordinate the infection process by triggering multiple responses in the plant root, such as calcium...

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Published in:Frontiers in plant science 2023-07, Vol.14, p.1152493-1152493
Main Authors: Parra-Aguilar, Thelma J, Sarmiento-López, Luis G, Santana, Olivia, Olivares, Juan Elías, Pascual-Morales, Edgar, Jiménez-Jiménez, Saul, Quero-Hostos, Andrea, Palacios-Martínez, Janet, Chávez-Martínez, Ana I, Cárdenas, Luis
Format: Article
Language:English
Subjects:
arbuscule
mycorrhization
nodulation
nodule
Plant Science
ROS
tetraspanin
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Summary:Arbuscular mycorrhizal (AM) fungi and rhizobia form two of the most important plant-microbe associations for the assimilation of phosphorus (P) and nitrogen (N). Symbiont-derived signals are able to coordinate the infection process by triggering multiple responses in the plant root, such as calcium influxes and oscillations, increased reactive oxygen species (ROS), cytoskeletal rearrangements and altered gene expression. An examination was made of the role of tetraspanins, which are transmembrane proteins that self-organize into tetraspanin web regions, where they recruit specific proteins into platforms required for signal transduction, membrane fusion, cell trafficking, and ROS generation. In plant cells, tetraspanins are scaffolding proteins associated with root radial patterning, biotic and abiotic stress responses, cell fate determination, plasmodesmata and hormonal regulation. Some plant tetraspanins, such as TETRASPANIN 8 and TETRASPANIN 9 (AtTET8 and AtTET9) are associated with exosomes during inter-kingdom communication. In this study, a homolog of , , in common bean ( var. Negro Jamapa) was examined in roots during interactions with and . The promoter of contained several acting regulatory DNA elements potentially related to mutualistic interactions, and was transcriptionally activated during AM fungal and rhizobial associations. Silencing it decreased the size and number of nodules, nitrogen fixation, and mycorrhizal arbuscule formation, whereas overexpressing it increased the size and number of nodules, and mycorrhizal arbuscule formation but decreased nitrogen fixation. appears to be an important element in both of these mutualistic interactions, perhaps through its interaction with NADPH oxidase and the generation of ROS during the infection processes.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2023.1152493