Digital light processing printing of non-modified protein-only compositions

• Published in: Materials today bio 2025-02, Vol.30, p.101384, Article 101384
• Main Authors: Bunin, Ayelet, Harari-Steinberg, Orit, Kam, Doron, Kuperman, Tatyana, Friedman-Gohas, Moran, Shalmon, Bruria, Larush, Liraz, Duvdevani, Shay I., Magdassi, Shlomo
• Format: Article
• Language: English
• Subjects: 3D printing; Cell-laden; di-tyrosine; Digital light processing (DLP); Non-modified
• ISSN: 2590-0064; 2590-0064
• DOI: 10.1016/j.mtbio.2024.101384

This study explores the utilization of digital light processing (DLP) printing to fabricate complex structures using native gelatin as the sole structural component for applications in biological implants. Unlike approaches relying on synthetic materials or chemically modified biopolymers, this research harnesses the inherent properties of gelatin to create biocompatible structures. The printing process is based on a crosslinking mechanism using a di-tyrosine formation initiated by visible light irradiation. Formulations containing gelatin were found to be printable at the maximum documented concentration of 30 wt%, thus allowing the fabrication of overhanging objects and open embedded. Cell adhesion and growth onto and within the gelatin-based 3D constructs were evaluated by examining two implant fabrication techniques: (1) cell seeding onto the printed scaffold and (2) printing compositions that contain cells (cell-laden). The preliminary biological experiments indicate that both the cell-seeding and cell-laden strategies enable making 3D cultures of chondrocytes within the gelatin constructs. The mechanical properties of the gelatin scaffolds have a compressive modulus akin to soft tissues, thus enabling the growth and proliferation of cells, and later degrade as the cells differentiate and form a grown cartilage. This study underscores the potential of utilizing non-modified protein-only bioinks in DLP printing to produce intricate 3D objects with high fidelity, paving the way for advancements in regenerative tissue engineering. This research explores non-modified, protein-only bioinks for digital light processing (DLP) printing. By harnessing the native gelatin properties, intricate 3D structures are fabricated. Visible light-induced crosslinking enables the fabrication of cell-laden and cell-seeded cartilage implants. [Display omitted]