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EFFECT OF OBESTATIN ON GLUCOSE HOMEOSTASIS, INSULIN RESISTANCE AND SERUM INSULIN LEVELS IN TYPE 2 DIABETIC RATS
Objective: To determine the effect of obestain administration on plasma glucose levels, serum insulin levels and insulin resistance in type 2 diabetic Sprague Dawely rats.Study Design: Randomized controlled trail.Place and Duration of Study: This study was carried out at Army Medical College Rawalpi...
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Published in: | Pakistan Armed Forces medical journal 2018-08, Vol.68 (4), p.998-1002 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | Objective: To determine the effect of obestain administration on plasma glucose levels, serum insulin levels and insulin resistance in type 2 diabetic Sprague Dawely rats.Study Design: Randomized controlled trail.Place and Duration of Study: This study was carried out at Army Medical College Rawalpindi, from April 2013 to July 2013.Material and Methods: This study was a randomized controlled trial conducted at Physiology department, Army Medical College. Forty-five healthy Sprague Dawley rats were randomly divided in to 3 groups i.e. control group (group-I) fed with normal pellet diet (NPD), diabetic group (group-II) and obestatin treated diabetic group (group-III) fed with high fat diet (HFD). Diabetic was induced by single intraperitoneal injection of streptozotocin (35mg/kg). Insulin resistance was determining by НОМА-IR. After Eight weeks, group-II was treated with obestatm (InmOl/100ml mtraperitoneally) Blood samples were obtained by terminal intracardic sampling for bioassays of plasma glucose by glucose oxidase method, serum insulin level by ELISA and measurement of НОМА-IR. Mean ± SD was calculated. Statistical significance of differences across the groups was determined by one-way ANOVA by followed by post Hoc Turkey's test. A p-value |
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ISSN: | 0030-9648 2411-8842 |