Expression and clinicopathologic significance of human achaete-scute homolog 1 in pulmonary neuroendocrine tumors

• Published in: Zhongguo fei ai za zhi 2010-04, Vol.13 (4), p.317-321
• Main Authors: Li, Fei, Zhong, Zhiyong, Li, Rui, Huang, Heyu, Wang, Lijun, Zheng, Donghan, Zhang, Daorong
• Format: Article
• Language: Chinese
• Subjects: Adenocarcinoma - genetics; Adenocarcinoma - metabolism; Adenocarcinoma - pathology; Basic Helix-Loop-Helix Transcription Factors - genetics; Basic Helix-Loop-Helix Transcription Factors - metabolism; Carcinoma, Large Cell - genetics; Carcinoma, Large Cell - metabolism; Carcinoma, Large Cell - pathology; Carcinoma, Neuroendocrine - genetics; Carcinoma, Neuroendocrine - metabolism; Carcinoma, Neuroendocrine - pathology; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Gene Expression Regulation, Neoplastic - genetics; Gene Expression Regulation, Neoplastic - physiology; Human achaete-scute homolog 1; Humans; Immunohistochemistry; Lung neoplasms; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Lung neuroendocrine neoplasms; Neuroendocrine Tumors - genetics; Neuroendocrine Tumors - metabolism; Neuroendocrine Tumors - pathology; Reverse Transcriptase Polymerase Chain Reaction; Small Cell Lung Carcinoma - genetics; Small Cell Lung Carcinoma - metabolism; Small Cell Lung Carcinoma - pathology; Tumor markers
• ISSN: 1009-3419; 1999-6187
• DOI: 10.3779/j.issn.1009-3419.2010.04.09

Human achaete-scute homolog 1 (hASH1) gene plays a critical role in development of the central nervous system, automatic nervous system, adrenal medullary chromaffin cells, thyroid C cells and pulmonary neuroendocrine cells. The aim of this study is to determine hASH1 gene expression in the normal lung tissue and various types of lung tumors, to analyze whether its expression correlated with pulmonary neuroendocrine markers, and to explore the possibility of hASH1 as clinical pathological markers in the neuroendocrine tumors compared with previous neuroendocrine tumor markers. hASH1, Chromogranin A, Synaptophysin and CD56 expression were examined in lung tumor specimens (lung inflammatory pseudotumor, squamous cell carcinoma, adenocarcinomas, large cell carcinoma, typical carcinoids, atypical carcinoids, large cell neuroendocrine carcinomas and small cell lung carcinoma and corresponding normal lung specimens) using immunohistochemistry (S-P method). Western blot and reverse transcription polymerase chain rea