Potential of RH5 Antisense on Plasmodium falciparum Proliferation Abatement

Infections by , are becoming increasingly difficult to treat. Therefore, there is an urgent need for novel antimalarial agents' discovery against infection. In present study, we described a 2'-O-Methyl gapmer phosphorothioate oligonucleotide antisense targeting translation initiation regio...

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Bibliographic Details
Published in:Iranian journal of parasitology 2022-10, Vol.17 (4), p.525-534
Main Authors: Razavi Vakhshourpour, Sepand, Nateghpour, Mehdi, Shahrokhi, Nader, Motevalli Haghi, Afsaneh, Mohebali, Mehdi, Hanifian, Haleh
Format: Article
Language:English
Subjects:
Antisense
Blood
Cell division
Gene expression
Genomes
Laboratories
Ligands
Malaria
Original
Parasites
Parasitology
Plasmodium falciparum
Proteins
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Summary:Infections by , are becoming increasingly difficult to treat. Therefore, there is an urgent need for novel antimalarial agents' discovery against infection. In present study, we described a 2'-O-Methyl gapmer phosphorothioate oligonucleotide antisense targeting translation initiation region of 3D7 strain gene. The study was conducted in Pasteur Institute of Iran in 2020. ODNs effects were measured by microscopic examination and real time RT-PCR. For microscopy, microplates were charged with 2'-OMe ODNs at different dilutions. Unsynchronized parasites were added to a total of 0.4 ml (0.4% parasitemia, 5% red blood cells), and slides were prepared. Proportion of infected cells was measured by counting at least 500 red blood cells. genes start codon regions selected as conserved region besed on alignment results. Gap- -As which was complementary to sequence surrounding AUG start codon significantly reduced parasite growth (>90% at 50 nM) compared to sense sequence control (Gap- -Se) (17%), (
ISSN:1735-7020
2008-238X
DOI:10.18502/ijpa.v17i4.11280