A root system architecture regulator modulates OsPIN2 polar localization in rice

• Published in: Nature communications 2025-01, Vol.16 (1), p.15-13, Article 15
• Main Authors: Li, Yong, Ren, Meiyan, Wu, Yunrong, Wang, Lingling, Zhao, Keju, Gao, Hongsheng, Li, Mengzhen, Liu, Yu, Zhu, Jianshu, Xu, Jiming, Mo, Xiaorong, Wu, Zhongchang, Lu, Chungui, Zheng, Shaojian, Mao, Chuanzao
• Format: Article
• Language: English
• Subjects: 38; 38/111; 38/35; 38/77; 42/44; 631/449/1736; 631/449/2653/1359; 631/449/448/2651; 82/51; Agricultural production; Auxins; Cell Membrane - metabolism; Cereal crops; Crop yield; Crops; Degradation; Flowering; Gene Expression Regulation, Plant; Gravitropism; Gravitropism - genetics; Growth patterns; Humanities and Social Sciences; Indoleacetic Acids - metabolism; Localization; multidisciplinary; Mutation; Nutrient uptake; Oryza - genetics; Oryza - growth & development; Oryza - metabolism; Phosphorylation; Plant growth; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - growth & development; Plant Roots - metabolism; Plants, Genetically Modified; Proteasome 26S; Proteasome Endopeptidase Complex - metabolism; Proteasomes; Rice; Rice fields; Roots; Science; Science (multidisciplinary); Seedlings; Seedlings - genetics; Seedlings - growth & development; Seedlings - metabolism; Ubiquitin-protein ligase; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; Ubiquitination
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-55324-5

Ideal root system architecture (RSA) is important for efficient nutrient uptake and high yield in crops. We cloned and characterized a key RSA regulatory gene, GRAVITROPISM LOSS 1 ( OsGLS1 ), in rice ( Oryza sativa L.). The gls1 mutant displays an increased root growth angle, longer primary roots, more adventitious roots and greater nutrient uptake efficiency and grain yield in paddy fields. OsGLS1 is strongly expressed in the root tips of seedlings and in leaves at the flowering stage. OsGLS1 encodes a RING finger E3 ubiquitin ligase mainly localizing at the basal plasma membrane (PM) in several root cell types when phosphorylated on its Ser-30 residue. OsGLS1 interacts with, ubiquitinates and promotes the degradation of basally localized PIN-FORMED 2 (OsPIN2) via the 26S proteasome, thus establishing the typical apical PM localization of OsPIN2 and polar auxin transport, ultimately shaping RSA. This previously unidentified OsGLS1-OsPIN2 regulatory pathway will contribute to an optimal RSA for enhancing nutrient efficiency in rice and other crops. Li et al. characterized the gene GRAVITROPISM LOSS 1 ( OsGLS1 ) in rice, which regulates root system architecture (RSA) to enhance nutrient uptake and yield. OsGLS1 promotes the degradation of OsPIN2, influencing auxin transport and root growth patterns.